Cloning and expression in Escherichia coli of pectinase genes of Erwinia carotovora subsp. carotovora
Cloning and expression in Escherichia coli of pectinase genes of Erwinia carotovora subsp. carotovora
复制标题
胡萝卜软腐欧文氏菌亚种果胶酶基因的克隆及在大肠杆菌中的表达。
DOI：
10.1128/aem.49.3.714-717.1985
复制
发表时间：
1985
影响因子：
4.4
通讯作者：
Zinkand Arun K. Chatterjee
中科院分区：
文献类型：
--
作者：
Zinkand Arun K. Chatterjee
编码胡萝卜软腐欧文氏菌用粘粒pHC79将胡萝卜软腐菌Ecc71克隆到大肠杆菌HB 101中。克隆的果胶酶基因的产物在等电迁移率、底物降解模式和浸渍马铃薯块茎组织的能力方面与菌株Ecc 71中的对应物相同。
Genes coding for an endo-pectate lyase, an exo-pectate lyase, and an endopolygalacturonase of Erwinia carotovora subsp. carotovora Ecc71 were cloned in Escherichia coli HB101, using the cosmid pHC79. The products of the cloned pectinase genes paralleled their counterparts in strain Ecc71 in isoelectric mobility, mode of substrate degradation, and ability to macerate potato tuber tissue.