USE OF [C-14] AMINO ACIDS TO STUDY SITES AND RATES OF ANTIBODY SYNTHESIS IN LIVING HYPERIMMUNE RABBITS
USE OF [C-14] AMINO ACIDS TO STUDY SITES AND RATES OF ANTIBODY SYNTHESIS IN LIVING HYPERIMMUNE RABBITS
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DOI：
10.1042/bj0680146
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发表时间：
1958-01-01
影响因子：
4.1
通讯作者：
SULITZEANU, BD
中科院分区：
文献类型：
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作者：
HUMPHREY, JH;SULITZEANU, BD
从静脉注射3型肺炎球菌超免疫的兔组织中回收细胞内抗体，并在给予C14标记的氨基酸后的不同时间间隔灌注无血。通过提前2-3天注射I131标记的抗体并使其与细胞外液中的抗体达到平衡，从而允许预先形成的细胞外抗体。C14标记的抗体在施用标记物的几分钟内在肺、骨髓、脾、淋巴腺和阑尾（但不在肝或肾中）中发现，并且在C14在血浆抗体中可检测之前。从组织中回收的细胞内抗体量为：肺X骨髓脾淋巴腺X阑尾。测定细胞内抗体和细胞内游离氨基酸的特异性Cl 4-活性的时间过程。根据结果，计算出肺中细胞内抗体的“周转时间”约为2小时。考虑到回收的细胞内抗体总量和维持观察到的血浆水平所需的抗体日产量，计算出所有组织抗体的平均“周转时间”约为3小时。在注射C14标记氨基酸混合物后的最初几分钟内，考虑血浆游离氨基酸比活性的时间过程表明（对于2.5kg的兔子），（1）细胞和组织液之间的氨基酸交换速率为100 mg/分钟的数量级，和（2）“细胞内游离氨基酸库”的大小发生这种快速交换的氨基酸库的数量级是血浆游离氨基酸库的100倍。
Intracellular antibody was recovered from tissues of rabbits hyperimmunized by intravenous injections of type 3 pneumococci, and perfused free of blood at various time intervals after administration of C14-labeled amino acids. Allowance was made for pre-formed extracellular antibody by injecting I131-labeled antibody 2-3 days beforehand and allowing it to come into equilibrium with antibody in the extracellular fluid. C14-labeled antibody was found in lungs, bone marrow, spleen, lymph glands and appendix (but not in liver or kidneys) within a few minutes of administering the label, and before C14 was detectable in plasma antibody. The amounts of intracellular antibody recovered from tissues were: lungXxbone marrowspleenlymph glands^appendix. The time course was determined of the specific Cl4-activities of intracellular antibody and of the intracellular free amino acids. From the results the "turnover time" of intracellular antibody in the lung was calculated to be of the order of 2 hours. From consideration of the total amounts of intracellular antibody recovered, and of the daily production of antibody required to maintain the observed plasma levels, the average "turnover time" of all tissue antibody was calculated to be of the order of 3 hours. Consideration of the time course of plasma-free amino acid specific activities during the first few minutes after injection of a mixture of C14-labeled amino acids suggests (for a 2.5 kg rabbit) that (1) the rate of exchange of amino acids between cells and tissue fluids is of the order of 100 mg/minute and (2) the size of the "intracellular free amino acid pool" with which this rapid exchange occurs is of the order of 100 times the plasma-free amino acid pool.