Expression profiles of miRNAs and involvement of miR-100 and miR-34 in regulation of cell cycle arrest in Artemia.
Expression profiles of miRNAs and involvement of miR-100 and miR-34 in regulation of cell cycle arrest in Artemia.
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DOI：
10.1042/bj20150116
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发表时间：
2015-09
期刊：
影响因子：
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通讯作者：
Ling-Ling Zhao-Ling;Feng Jin;Xiang Ye;Lin Zhu;Jin-Shu Yang;Wei-Jun Yang
中科院分区：
文献类型：
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作者：
Ling-Ling Zhao-Ling;Feng Jin;Xiang Ye;Lin Zhu;Jin-Shu Yang;Wei-Jun Yang
细胞周期的调节是复杂的，但对正常发育、繁殖和抗应激至关重要。为了在不利的环境条件下生存，甲壳动物卤虫产生滞育胚胎，其代谢维持在极低的水平。在本研究中，miRNAs的表达谱在卤虫滞育进入和终止的特点是使用高通量测序。共鉴定出13个未分类的miRNAs和370个miRNAs，分属于87个家族，其中107个miRNAs在进入滞育和解除滞育过程中差异表达。我们重点研究了其中两种miRNAs，miR-100和miR-34，在调节细胞周期进程中的作用;在进入滞育的各个阶段，这些miRNAs表现出相反的表达模式。功能分析显示，miR-100和miR-34分别通过靶向polo样激酶1（PLK 1），激活丝裂原活化蛋白激酶激酶-细胞外信号调节激酶-核糖体S6激酶2（MEK-ERK-RSK 2）通路和细胞周期蛋白K（cyclin K），抑制RNA聚合酶II（RNAP II）活性，从而调节滞育进入期间的细胞周期。本研究中的发现提供了对miR-100和miR-34功能的深入了解，并表明miRNAs在卤虫中的表达谱可用于表征其在细胞周期调控中的功能。
Regulation of the cell cycle is complex but critical for proper development, reproduction and stress resistance. To survive unfavourable environmental conditions, the crustacean Artemia produces diapause embryos whose metabolism is maintained at extremely low levels. In the present study, the expression profiles of miRNAs during Artemia diapause entry and termination were characterized using high-throughput sequencing. A total of 13 unclassified miRNAs and 370 miRNAs belonging to 87 families were identified; among them, 107 were differentially expressed during diapause entry and termination. We focused on the roles of two of these miRNAs, miR-100 and miR-34, in regulating cell cycle progression; during the various stages of diapause entry, these miRNAs displayed opposing patterns of expression. A functional analysis revealed that miR-100 and miR-34 regulate the cell cycle during diapause entry by targeting polo-like kinase 1 (PLK1), leading to activation of the mitogen-activated protein kinase kinase-extracellular signal-regulated kinase-ribosomal S6 kinase 2 (MEK-ERK-RSK2) pathway and cyclin K, leading to suppression of RNA polymerase II (RNAP II) activity respectively. The findings presented in the present study provide insights into the functions of miR-100 and miR-34 and suggest that the expression profiles of miRNAs in Artemia can be used to characterize their functions in cell cycle regulation.