Bone Properties by Nanoindentation in Mild and Severe Bone Properties by Nanoindentation in Mild and Severe Osteogenesis Imperfecta
Bone Properties by Nanoindentation in Mild and Severe Bone Properties by Nanoindentation in Mild and Severe Osteogenesis Imperfecta
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发表时间：
2021
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通讯作者：
C. Albert
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作者：
C. Albert
研究背景成骨不全是一种以骨脆性为特征的异质性遗传性疾病。以前的研究表明，受损的胶原网络和异常矿化影响骨组织的性质，然而，很少有数据可用于描述患有这种疾病的个体的骨材料性质。骨材料特性尚未在具有最常见的成骨类型I的个体中表征。方法采用纳米压痕法对11例儿童成骨不全患者常规手术截骨标本的骨组织弹性模量和硬度进行测量。这些属性进行了比较成骨不全类型I（轻度，n = 6）和III（严重，n = 5），以及使用线性混合模型分析之间的间质和骨的微观结构区域。结果疾病严重程度类型对弹性模量（7%，P = 0.02）和硬度（8%，P < 0.01）的影响较小，但具有统计学显著性。与成骨肌I型的个人有较高的模量和硬度比那些与III型。总体而言，间质板层骨区域的平均模量和硬度值比骨细胞区域高13%（P < 0.001）。目前的研究提出了第一个数据集，描述了具有最常见的成骨形式的个体的骨材料特性，即，I型。结果表明，内在的骨组织特性受表型的影响。了解成骨过程中骨的材料特性将有助于开发模型以帮助预测骨折风险。
Background Osteogenesis imperfecta is a heterogeneous genetic disorder characterized by bone fragility. Previous research suggests that impaired collagen network and abnormal mineralization affect bone tissue properties, however, little data is yet available to describe bone material properties in individuals with this disorder. Bone material properties have not been characterized in individuals with the most common form of osteogenesis imperfecta , type I. Methods Bone tissue elastic modulus and hardness were measured by nanoindentation in eleven osteotomy specimens that were harvested from children with osteogenesis imperfecta during routine surgeries. These properties were compared between osteogenesis imperfecta types I (mild, n = 6) and III (severe, n = 5), as well as between interstitial and osteonal microstructural regions using linear mixed model analysis. Findings Disease severity type had a small but statistically significant effect on modulus (7%, P = 0.02) and hardness (8%, P < 0.01). Individuals with osteogenesis imperfecta type I had higher modulus and hardness than did those with type III. Overall, mean modulus and hardness values were 13% greater in interstitial lamellar bone regions than in osteonal regions ( P < 0.001). The current study presents the first dataset describing bone material properties in individuals with the most common form of osteogenesis imperfecta , i.e., type I. Results indicate that intrinsic bone tissue properties are affected by phenotype. Knowledge of the material properties of bones in osteogenesis imperfecta will contribute to the ability to develop models to assist in predicting fracture risk.