The small intestine proteome is changed in preterm pigs developing necrotizing enterocolitis in response to formula feeding
The small intestine proteome is changed in preterm pigs developing necrotizing enterocolitis in response to formula feeding
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DOI：
10.1093/jn/138.10.1895
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发表时间：
2008-10-01
影响因子：
4.2
通讯作者：
Wan, Jennifer Man-Fan
中科院分区：
文献类型：
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作者：
Jiang, Pingping;Siggers, Jayda Lee Ann;Wan, Jennifer Man-Fan
坏死性小肠结肠炎（NEC）是新生儿早产儿最常见的胃肠道急症。临床研究表明，肠内配方奶粉喂养的早产儿NEC的发病率增加;然而，发病机制仍不清楚。为了鉴定NEC相关蛋白的分子机制，我们应用蛋白质组学分析来表征新生早产仔猪肠内配方奶粉喂养24 h后NEC的蛋白质表达谱的变化。蛋白质表达的变化进行了鉴定，使用二维凝胶电泳和肽质量指纹与MS以及蛋白质印迹分析。鉴定了19个差异表达的蛋白质，这些蛋白质在氧化应激、分子伴侣、信号转导、蛋白质折叠和降解、氧转运、信号转导和能量代谢中起作用。水平增加的蛋白质包括含锰超氧化物歧化酶和血红蛋白亚基，而表达减少的蛋白质包括山梨醇脱氢酶、线粒体醛脱氢酶2、葡萄糖调节蛋白75、CRY蛋白、snail同系物3、甲状腺激素结合蛋白前体、和DJ 1（帕金森氏病7）等。这些数据提供了新的机制的见解NEC的发病机制和侮辱的配方饮食的过早肠道。
Necrotizing enterocolitis (NEC) is the most common gastrointestinal emergency in newborn premature infants. Clinical studies show increased incidence of NEC in premature infants with enteral formula feeding; however, pathogenesis remains unclear. To identify the NEC-related proteins for molecular mechanisms, we applied proteomics analysis to characterize changes in the protein expression profile of newborn premature piglet intestines with NEC developed after enteral formula feeding for 24 h. Changes in protein expression were identified using 2-dimensional gel electrophoresis and peptide mass fingerprinting with MS as well as western blotting analysis. Nineteen differentially expressed proteins were identified and these have roles in oxidative stress, chaperone, signal transduction, protein folding and degradation, oxygen transport, signal transduction, and energy metabolism. Proteins with increased levels include manganese-containing superoxide dismutase and hemoglobin subunit and proteins with decreased expression include sorbitol dehydrogenase, mitochondrial aldehyde dehydrogenase 2, glucose-regulated protein 75, CRY protein, snail homolog 3, thyroid hormone-binding protein precursor, and DJ1 (Parkinson's disease 7) etc. The data provided novel mechanistic insights into the pathogenesis of NEC and the insults of a formulated diet to the premature gut.