Continuous minimally-invasive alcohol monitoring using microneedle sensor arrays.
Continuous minimally-invasive alcohol monitoring using microneedle sensor arrays.
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DOI：
10.1016/j.bios.2017.01.016
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发表时间：
2017-05-15
影响因子：
12.6
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Mohan AMV;Windmiller JR;Mishra RK;Wang J
本工作描述了一种有吸引力的皮肤佩戴的微针传感装置，用于皮下酒精的微创电化学监测。该装置由与Pt和Ag线集成的金字塔形微针结构的组件组成，每个微针结构具有微腔开口。通过将邻苯二胺电聚合到铂丝微换能器上，然后将醇氧化酶（AOx）固定在中间壳聚糖层中，沿着与外部Nafion层一起来修改微针孔径。所得的微针为基础的酶电极显示在人工组织液中的无干扰乙醇检测，而不影响其灵敏度，稳定性和响应时间。通过离体小鼠皮肤模型分析证实了皮肤渗透能力和生物传感器性能对皮下酒精监测的有效性。我们的研究结果表明，新的微针传感器在现实生活中的连续非侵入性酒精监测具有相当大的前景。
The present work describes an attractive skin-worn microneedle sensing device for the minimally invasive electrochemical monitoring of subcutaneous alcohol. The device consists of an assembly of pyramidal microneedle structures integrated with Pt and Ag wires, each with a microcavity opening. The microneedle aperture was modified by electropolymerizing o-phenylene diamine onto the Pt wire microtransducer, followed by the immobilization of alcohol oxidase (AOx) in an intermediate chitosan layer, along with an outer Nafion layer. The resulting microneedle-based enzyme electrode displays an interference-free ethanol detection in artificial interstitial fluid without compromising its sensitivity, stability and response time. The skin penetration ability and the efficaciousness of the biosensor performance towards subcutaneous alcohol monitoring was substantiated by the ex vivo mice skin model analysis. Our results reveal that the new microneedle sensor holds considerable promise for continuous non-invasive alcohol monitoring in real-life situations.