CD146+ skeletal stem cells from growth plate exhibit specific chondrogenic differentiation capacity in vitro
CD146+ skeletal stem cells from growth plate exhibit specific chondrogenic differentiation capacity in vitro
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来自生长板的CD146骨骼干细胞在体外表现出特定的软骨形成分化能力
DOI：
10.3892/mmr.2017.7616
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发表时间：
2017-09
影响因子：
3.4
通讯作者：
Guo Fengjing
中科院分区：
文献类型：
--
作者：
Wu Yingxing;Jing Xingzhi;Sun Yue;Ye Yaping;Guo Jiachao;Huang Junming;Xiang Wei;Zhang Jiaming;Guo Fengjing
骨骼干细胞（SSC）是一群祖细胞，其产生出生后的骨骼组织，包括骨、软骨和骨髓基质，但不产生脂肪、造血或肌肉组织。生长板软骨细胞具有持续增殖和分化的能力，有助于生理性生长的持续进行。已经假设生长板含有SSC，其表现出期望的分化能力以产生骨和软骨。由于生长板软骨细胞的异质性，生长板中的SSC没有得到很好的研究。本研究使用分化簇（CD）146和CD 105作为标记物分离纯化的精原干细胞。使用磁激活细胞分选方法分离CD105+ SSC和CD146+ SSC。为了定量研究细胞的增殖和分化能力，将CD105+ SSCs和CD146+ SSCs的集落形成效率（CFE）和多向分化能力与未分选的细胞和脂肪源性干细胞（ASCs）进行比较。结果表明，CD 105+和CD 146+亚群代表了生成软骨细胞和骨细胞的SSC亚群，但不是脂肪细胞。与CD105+细胞和ASCs相比，CD146+细胞在体外表现出更大的CFE和持续的高软骨分化能力。因此，本研究表明，CD 146+亚群代表软骨谱系限制的SSC亚群，因此可能作为软骨再生的有价值的细胞来源。
Skeletal stem cells (SSCs) are a population of progenitor cells which give rise to postnatal skeletal tissues including bone, cartilage and bone marrow stroma, however not to adipose, haematopoietic or muscle tissue. Growth plate chondrocytes exhibit the ability of continuous proliferation and differentiation, which contributes to the continuous physiological growth. The growth plate has been hypothesized to contain SSCs which exhibit a desirable differentiation capacity to generate bone and cartilage. Due to the heterogeneity of the growth plate chondrocytes, SSCs in the growth plate are not well studied. The present study used cluster of differentiation (CD)146 and CD105 as markers to isolate purified SSCs. CD105+ SSCs and CD146+ SSCs were isolated using a magnetic activated cell sorting method. To quantitatively investigate the proliferation and differentiation ability, the colony-forming efficiency (CFE) and multilineage differentiation capacity of CD105+ SSCs and CD146+ SSCs were compared with unsorted cells and adipose-derived stem cells (ASCs). It was revealed that CD105+ and CD146+ subpopulations represented subsets of SSCs which generated chondrocytes and osteocytes, however not adipocytes. Compared with CD105+ subpopulations and ASCs, the CD146+ subpopulation exhibited a greater CFE and continuous high chondrogenic differentiation capacity in vitro. Therefore, the present study suggested that the CD146+ subpopulation represented a chondrolineagerestricted subpopulation of SSCs and may therefore act as a valuable cell source for cartilage regeneration.
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影响因子：
20.3
作者：
Balakumaran, Arun;Mishra, Prasun J.;Robey, Pamela G.
通讯作者：
Robey, Pamela G.
影响因子：
7.5
作者：
Wu CC;Liu FL;Sytwu HK;Tsai CY;Chang DM
通讯作者：
Chang DM
人骨髓基质细胞亚群的前瞻性隔离：富含Stro的1-，CD146-和CD105富含种群之间的比较研究。
影响因子：
8.2
作者：
Gothard D;Greenhough J;Ralph E;Oreffo RO
通讯作者：
Oreffo RO
影响因子：
3.3
作者：
Erdman, Christopher P.;Dosier, Christopher R.;Boyan, Barbara D.
通讯作者：
Boyan, Barbara D.
DOI：
--
发表时间：
2016
期刊：
--
影响因子：
--
作者：
Yangzi Jiang;Youzhi Cai;Wei Zhang-;Zi Yin;Changchang Hu;Tong Tong-Tong;Ping Lu;Shufang Zhang;D. Neculai;R. Tuan;H. Ouyang
通讯作者：
Yangzi Jiang;Youzhi Cai;Wei Zhang-;Zi Yin;Changchang Hu;Tong Tong-Tong;Ping Lu;Shufang Zhang;D. Neculai;R. Tuan;H. Ouyang