EFFECT OF DIETARY LIPIDS ON PLASMA ACTIVITY AND HEPATIC MESSENGER-RNA LEVELS OF CHOLESTERYL ESTER TRANSFER PROTEIN IN HIGH-RESPONDING AND LOW-RESPONDING BABOONS (PAPIO SPECIES)
EFFECT OF DIETARY LIPIDS ON PLASMA ACTIVITY AND HEPATIC MESSENGER-RNA LEVELS OF CHOLESTERYL ESTER TRANSFER PROTEIN IN HIGH-RESPONDING AND LOW-RESPONDING BABOONS (PAPIO SPECIES)
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DOI：
10.1016/0026-0495(94)90181-3
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发表时间：
1994-08-01
影响因子：
9.8
通讯作者：
MCGILL, HC
中科院分区：
文献类型：
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作者：
KUSHWAHA, RS;REARDON, CA;MCGILL, HC
我们比较了膳食胆固醇、脂肪类型（椰子油v玉米油）和表型（低低密度脂蛋白[LDL]反应v高LDL反应）对胆固醇酯转移蛋白（CETP）血浆活性和肝脏mRNA水平的影响。在交叉设计中，8只高LDL反应和8只低LDL反应狒狒被喂食一系列胆固醇含量逐渐增加（0.05、0.15、0.45和1.35毫克/千卡）的饮食，其中包括椰子油或玉米油。所有饮食均喂养 7 周。每种饮食 6 周后，测量血浆和脂蛋白胆固醇浓度、血浆卵磷脂：胆固醇酰基转移酶 (LCAT) 和 CETP 活性，以及 CETP 和载脂蛋白 (apo) A-I 的肝脏 mRNA 水平。分两步分析数据，即从食物到每种脂肪中胆固醇的初始变化的影响以及每种脂肪中胆固醇从0.05到1.35毫克/千卡逐步增加的影响。正如预期的那样，高反应狒狒在所有膳食胆固醇水平下，血浆低密度脂蛋白胆固醇都有更明显的增加，特别是以椰子油作为膳食脂肪时。血浆高密度脂蛋白 2 (HDL2) 和 HDL3 胆固醇随着椰子油和玉米油饮食中膳食胆固醇的增加而增加，高反应狒狒的增加幅度大于低反应狒狒。膳食胆固醇的逐步增加增加了喂食椰子油饮食的高反应和低反应狒狒的血浆 LCAT 活性，但喂食玉米油饮食的狒狒则没有。膳食胆固醇，无论脂肪类型如何，都会增加血浆 CETP 活性。高反应狒狒的 LCAT 和 CETP 活性增加幅度大于低反应狒狒。膳食胆固醇会增加两种类型脂肪的肝脏 CETP mRNA 水平，但当用椰子油喂养胆固醇时，高反应者的增加也比低反应者更大。因此，膳食胆固醇摄入量、饱和脂肪酸摄入量和高反应表型都与 CETP 活性增加相关。在用椰子油喂养的动物中，CETP活性的增加可能至少部分归因于该蛋白质的肝脏合成和分泌的增加。这可能有助于促进这些动物的促动脉粥样硬化表型。
We compared the effects of dietary cholesterol, type of fat (coconut oilvcorn oil), and phenotype (low low-density lipoprotein [LDL] responsevhigh LDL response) on the plasma activity and hepatic mRNA levels of cholesteryl ester transfer protein (CETP). In a crossover design, eight high- and eight low-LDL-responding baboons were fed a series of diets with increasing amounts of cholesterol (0.05, 0.15, 0.45, and 1.35 mg/kcal) with either coconut oil or corn oil. All diets were fed for 7 weeks each. Plasma and lipoprotein cholesterol concentrations, plasma lecithin:cholesterol acyltransferase (LCAT) and CETP activity, and hepatic mRNA levels for CETP and apolipoprotein (apo) A-I were measured after 6 weeks on each diet. Data were analyzed in two steps, ie, the effect of the initial change from chow to 0.05 mg cholesterol with each fat and the effect of the stepwise increase in cholesterol from 0.05 to 1.35 mg/kcal with each fat. High-responding baboons, as expected, showed a more pronounced increment in plasma LDL cholesterol at all dietary cholesterol levels, particularly with coconut oil as the dietary fat. Plasma high-density lipoprotein 2 (HDL2) and HDL3cholesterol increased as dietary cholesterol increased on both the coconut and corn oil diets, with a greater increase in high-responding baboons than in low-responding baboons. The stepwise increase in dietary cholesterol increased plasma LCAT activity in both high- and low-responding baboons fed the coconut oil diet, but not in those fed the corn oil diet. Dietary cholesterol, regardless of type of fat, increased plasma CETP activity. The increase in LCAT and CETP activities was greater in high-responding baboons than in low-responding baboons. Dietary cholesterol increased hepatic CETP mRNA levels with both types of fat, but the increase was also greater in high responders than in low responders when cholesterol was fed with coconut oil. Thus, dietary cholesterol intake, saturated fatty acid intake, and high-responding phenotype are all associated with increased CETP activity. The increase in CETP activity is probably attributable at least in part and in the coconut oil-fed animals to an increase in the hepatic synthesis and secretion of this protein. This may help to promote a proatherogenic phenotype in these animals.