DNA breakage upon K-shell excitation of phosphorus as a model for direct effects in radiation biology.
DNA breakage upon K-shell excitation of phosphorus as a model for direct effects in radiation biology.
复制标题
磷的 K 壳层激发引起的 DNA 断裂作为辐射生物学直接影响的模型。
作者：
C. Le Sech;H. Frohlich;C. Saint;M. Charlier
用超螺旋质粒研究了磷K壳共振吸收诱导DNA单链断裂和双链断裂。SSB和DSBs的生产动力学表现出线性和二次依赖，分别对光子注量。测量了截面和量子产率。磷原子的共振光激发被发现增加DSB/SSB比相比，非共振激发。该增强因子可与在文献中先前报道的类似实验条件下测得的酵母中细胞死亡和基因突变速率的增强有关。这种属于DNA的特定原子的共振激发被证明是研究纯直接效应的一种优雅方法。
Single-strand breaks (SSBs) and double-strand breaks (DSBs) induced in DNA under phosphorus K-shell resonant absorption have been studied using supercoiled plasmids. The kinetics of the production of SSBs and DSBs exhibits a linear and a quadratic dependence, respectively, on photon fluence. Cross sections and quantum yields have been measured. The resonant photoexcitation of the phosphorus atoms was found to increase the DSB/SSB ratio compared to the off-resonance excitation. This enhancement factor can be related to the measured enhancement of the rate of cellular death and gene mutation in yeast under similar experimental conditions reported previously in the literature. Such resonant excitation of a specific atom belonging to DNA turns out to be an elegant method to investigate pure direct effects.
影响因子：
5.6
作者：
RAHMOUNI, AR;WELLS, RD
通讯作者：
WELLS, RD