ANTIBACTERIAL AND ANTIMALARIAL PROPERTIES OF PEPTIDES THAT ARE CECROPIN-MELITTIN HYBRIDS
ANTIBACTERIAL AND ANTIMALARIAL PROPERTIES OF PEPTIDES THAT ARE CECROPIN-MELITTIN HYBRIDS
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DOI：
10.1016/0014-5793(89)81505-4
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发表时间：
1989-12-18
期刊：
影响因子：
3.5
通讯作者：
MERRIFIELD, RB
中科院分区：
文献类型：
--
作者：
BOMAN, HG;WADE, D;MERRIFIELD, RB
采用固相合成法制备了5种含有抗菌肽天蚕素A和蜂毒肽蜂毒肽序列的杂合肽。这些嵌合肽中的四个对代表性革兰氏阴性和革兰氏阳性细菌物种显示出良好的抗菌活性。最佳杂交体天蚕素A（1-13）-蜂毒素（1-13）对金黄色葡萄球菌的活性是天蚕素A的100倍。它也是比天蚕素B或爪蟾抗菌肽2好10倍的抗疟药。绵羊红细胞溶解蜂毒肽在低浓度，但不是由杂交分子，即使在50倍高浓度。
Solid phase synthesis was used to produce 5 hybrid peptides containing sequences from the antibacterial peptide, cecropin A, and from the bee venom toxin, melittin. Four of these chimeric peptides showed good antibacterial activity against representative Gram-negative and Gram-positive bacterial species. The best hybrid, cecropin A(1–13)-melittin(1–13) was 100-fold more active than cecropin A againstStaphylococcus aureus. It was also a 10-fold better antimalarial agent than cecropin B or magainin 2. Sheep red cells were lysed by melittin at low concentrations, but not by the hybrid molecules, even at 50 times higher concentrations.