Spatial single cell metabolomics: Current challenges and future developments.
Spatial single cell metabolomics: Current challenges and future developments.
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DOI：
10.1016/j.cbpa.2023.102327
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发表时间：
2023-05
影响因子：
7.8
通讯作者：
Kyle D G Saunders;H. Lewis;D. Beste;Olivier N. F. Cexus;Michael C. Bailey
中科院分区：
文献类型：
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作者：
Kyle D G Saunders;H. Lewis;D. Beste;Olivier N. F. Cexus;Michael C. Bailey
单细胞代谢组学是生物分析化学的一个快速发展的领域，旨在尽可能详细地观察细胞生物学。质谱成像和选择性细胞采样（例如使用纳米毛细管）是该领域的两种常见方法。最近的成就，例如细胞间相互作用的观察、脂质决定细胞状态和快速表型识别，证明了这些方法的有效性和该领域的发展势头。然而，只有满足该领域的普遍挑战，例如缺乏标准化和量化策略以及缺乏特异性/敏感性，单细胞代谢组学才能以同样的动力继续发展。质谱成像和选择性细胞采样具有独特的优势和挑战，在许多情况下是相互补充的。我们在此建议，通过推动这些方法的两个社区之间的合作，可以缓解每种方法特有的挑战。
Single cell metabolomics is a rapidly advancing field of bio-analytical chemistry which aims to observe cellular biology with the greatest detail possible. Mass spectrometry imaging and selective cell sampling (e.g. using nanocapillaries) are two common approaches within the field. Recent achievements such as observation of cell–cell interactions, lipids determining cell states and rapid phenotypic identification demonstrate the efficacy of these approaches and the momentum of the field. However, single cell metabolomics can only continue with the same impetus if the universal challenges to the field are met, such as the lack of strategies for standardisation and quantification, and lack of specificity/sensitivity. Mass spectrometry imaging and selective cell sampling come with unique advantages and challenges which, in many cases are complementary to each other. We propose here that the challenges specific to each approach could be ameliorated with collaboration between the two communities driving these approaches.