Contour area filtering of two-dimensional electrophoresis images.
Contour area filtering of two-dimensional electrophoresis images.
复制标题
二维电泳图像的轮廓区域过滤。
DOI：
10.1016/j.media.2006.01.004
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Wenger,Rephael
中科院分区：
文献类型：
--
作者：
Kazhiyur-Mannar,Ramakrishnan;Smiraglia,DominicJ;Plass,Christoph;Wenger,Rephael
本文提出了一种灰度图像前景和背景分离的算法--轮廓区域滤波。该算法是基于灰度轮廓线所包含的面积。它可以被看作是一种局部阈值，或作为种子生长算法，或作为一种分水岭分割。该算法的最大特点是利用目标区域来确定分割。因此，它相对不受图像上或不同图像之间的亮度和对比度变化的影响。轮廓区域过滤是专门为2D电泳凝胶的图像分析而设计的，尽管它可以应用于其他灰度图像。典型的凝胶图像是代表蛋白质或DNA限制性片段的1000-2500个点的荧光图像或荧光图像。图像是定量的，斑点强度反映了蛋白质的数量或DNA片段的拷贝数。背景强度可以在图像上广泛地变化，这是由斑点密度的变化和产生凝胶的物理实验室过程引起的。分析和比较凝胶图像需要提取和分割斑点，配准图像和匹配斑点，并测量斑点之间的差异。实验结果表明，轮廓区域滤波是一种快速、有效的电泳凝胶背景与前景分离方法，具有极高的准确性。
We describe an algorithm, Contour Area Filtering, for separating background from foreground in gray scale images. The algorithm is based on the area contained within gray scale contour lines. It can be viewed as a form of local thresholding, or as a seed growing algorithm, or as a type of watershed segmentation. The most important feature of the algorithm is that it uses object area to determine the segmentation. Thus, it is relatively impervious to brightness and contrast variations across an image or between different images. Contour Area Filtering was designed specifically for image analysis of 2D electrophoresis gels, although it can be applied to other gray scale images. A typical gel image is an electrophoretogram or a phosphor image of 1000–2500 spots representing protein or DNA restriction fragments. The images are quantitative with spot intensities reflective of the number of proteins or the DNA fragment copy number. The background intensity can vary widely across the image caused both by variation in spot density and by the physical laboratory process of creating a gel. Analyzing and comparing gel images entails extracting and segmenting spots, registering images and matching spots, and measuring differences between spots. We present experimental results which show that Contour Area Filtering is a quick, efficient method for separating electrophoresis gel background from foreground with extremely high accuracy.