QUANTITATIVE-ANALYSIS OF HYALURONAN IN VITREOUS-HUMOR USING CAPILLARY ELECTROPHORESIS
QUANTITATIVE-ANALYSIS OF HYALURONAN IN VITREOUS-HUMOR USING CAPILLARY ELECTROPHORESIS
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DOI：
10.1002/elps.11501501137
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发表时间：
1994-07-01
期刊：
影响因子：
2.9
通讯作者：
ARCHER, D
中科院分区：
文献类型：
--
作者：
GRIMSHAW, J;KANE, A;ARCHER, D
采用毛细管电泳法分离和定量分析人和牛玻璃体中的糖胺聚糖透明质酸，在200 nm处测定吸光度。使用由已知浓度的脐带来源的透明质酸组成的标准品进行校准。通过H-1 NMR（核磁共振）检查标准品的纯度。浓度低至25微克/毫升，可以检测到毛细管电泳。通过透明质酸酶解聚天然粘多糖，确认信号是由于透明质酸引起的。这导致透明质酸峰的损失和对应于具有较短迁移时间的寡聚片段的几个新峰的出现。毛细管电泳是一种可重复且灵敏的技术，用于玻璃体样本中透明质酸的定量和表征。
Capillary electrophoresis was used for the separation and quantitative analysis of the glycosaminoglycan hyaluronan in human and bovine vitreous with detection by W absorbance at 200 nm. Calibration was carried out using standards made up from known concentrations of hyaluronan of umbilical cord origin. The purity of the standard was examined by H-1 NMR (nuclear magnetic resonance). Concentrations as low as 25 mu g/mL could be detected by capillary electrophoresis. Confirmation that the signal was due to hyaluronan was obtained by depolymerization of the native mucopolysaccharide by hyaluronidase. This resulted in loss of the hyaluronan peak and appearance of several new peaks corresponding to the oligomeric fragments which had shorter migration times. Capillary electrophoresis is a reproducible and sensitive technique for the quatification and characterisation of hyaluronan in vitreous samples.