The production of new hydranths in Hydra by the insertion of small grafts
The production of new hydranths in Hydra by the insertion of small grafts
复制标题
DOI：
10.1002/jez.1400070102
复制
发表时间：
1909-08-01
影响因子：
--
通讯作者：
Browne, EN
中科院分区：
文献类型：
--
作者：
Browne, EN
1906-1908年冬，我进行了水螅嫁接再生的试验，目的是进一步了解水螅再生的有关因素。这项工作是在摩根教授的建议下完成的，我真诚地感谢他的关心和帮助。在我的第一个系列实验中，我用普通的绿色水螅做实验，我试图发现什么材料嫁接后会产生必要的刺激，促使新水螅的发展。实验发现，如果将基部带有少量口唇组织的触手插入另一水螅体内，则该水螅在嫁接处会再生出一个全新的水螅，而嫁接的触手仍是新的触手环之一。问题是，再生是否仅仅是由于任何种类的外来组织的存在，或者它是否是由某种特殊的物质引发的;如果是这样，这是什么样的组织。为了解决这个问题，从水螅身体的不同区域取出组织，并移植到同一物种的其他水螅身体的不同区域。在我的第二系列实验中，我致力于找出再生材料的确切来源。一个极好的机会来决定这个问题是由先生DD惠特尼的发现，绿色的颜色可以完全消除水螅绿色，把动物在一个。5%的甘油溶液，并将其放置约三周。这些人工
During the winters of 1906-1908, I carried on some experiments in grafting Hydra viridis for the purpose of throwing more light on the factors concerned in regeneration. The work was done at the suggestion of Professor Morgan, whom I sincerely thank for his kind interest and help.In my first series of experiments, which were done with the ordinary green hydra, I tried to discover what materialwhen grafted would give, the necessary stimulus to call forth the development of a new hydranth. It was found by experiment that if a tentacle with a small bit of peristome tissue at its base was inserted into the body of another hydra, the stock would regenerate a whole new hydranth at the place of grafting, the grafted tentacle remaining as one of the new circlet of tenacles. The question arose whether the regeneration was due merely to the presence of foreign tissue of any kind or whether it was initiated by some special kind of material; and if so, what kind of tissue this was. T o solve this problem, tissue was taken from different regions of the body of Hydra viridis and grafted into different regions of the body of other hydras of the same species. In my second series of experiments, I endeavored to find out the exact origin of the regenerating material. An excellent opportunity to decide this question was offered by the discovery of Mr. DD Whitney that the green color can be entirely removed from Hydra viridis by putting the animals in a. 5 per cent glycerine solution and leaving them for about three weeks. These artificial