RAPID CHANGES IN STRIATAL ASCORBATE IN RESPONSE TO TAIL-PINCH MONITORED BY CONSTANT POTENTIAL VOLTAMMETRY
RAPID CHANGES IN STRIATAL ASCORBATE IN RESPONSE TO TAIL-PINCH MONITORED BY CONSTANT POTENTIAL VOLTAMMETRY
复制标题
DOI：
10.1016/0306-4522(89)90349-7
复制
发表时间：
1989-01-01
期刊：
影响因子：
3.3
通讯作者：
FILLENZ, M
中科院分区：
文献类型：
--
作者：
BOUTELLE, MG;SVENSSON, L;FILLENZ, M
用线性扫描和碳糊电极植入大鼠纹状体所记录的伏安图中的第一个峰是由于抗坏血酸的氧化。当电位保持在略高于该峰值的水平时，记录电流，该电流通过在电极附近微量注射抗坏血酸氧化酶而消除；这表明这是由于抗坏血酸的氧化。该电流显示出与抗坏血酸峰值大小相同的日变化，其上升和下降与运动活动的开始和抵消一致。用回形针夹住尾部，会使抗坏血酸电流立即上升，而回形针一拿开，电流就开始下降。恒电位下抗坏血酸电流的测量为监测细胞外脑抗坏血酸在生理刺激下的快速变化提供了一种技术。
The first peak in the voltammogram recorded with linear sweep and a carbon paste electrode implanted in the rat striatum is due to the oxidation of ascorbic acid. When the potential is held at a level slightly positive to this peak a current is recorded which is abolished by the microinjection of ascorbic acid oxidase in the vicinity of the electrode; this suggests that it is due to oxidation of ascorbate. This current shows the same diurnal variation as the size of the ascorbate peak and its rise and fall coincides with the onset and offset of motor activity. A tail-pinch applied through a paper clip causes an immediate rise in the ascorbate current which begins to fall as soon as the paper clip is removed. Measurement of the ascorbate current at constant potential provides a technique for monitoring rapid changes in extracellular brain ascorbate in response to physiological stimuli.