Responses of immunocompetent cells in the dental pulp to replantation during the regeneration process in rat molars
Responses of immunocompetent cells in the dental pulp to replantation during the regeneration process in rat molars
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DOI：
10.1007/s004410000263
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发表时间：
2000-11-01
影响因子：
3.6
通讯作者：
Ohshima, H
中科院分区：
文献类型：
--
作者：
Shimizu, A;Nakakura-Ohshima, K;Ohshima, H
采用免疫细胞化学方法，结合高碘酸-席夫(PAS)组织化学法，研究了大鼠磨牙牙髓再生过程中牙髓再生过程中免疫活性细胞对再植的反应。再植后牙髓中OX6和ED1阳性细胞的数量和免疫反应强度均增加，但几乎所有的PGP9.5免疫反应神经在再植初期均减弱。术后第3天，成功者牙髓-牙本质边界有大量OX6和ED1免疫阳性细胞聚集，胞质突起伸入牙本质小管。术后第7天修复性牙本质形成后，OX6和ED1免疫阳性细胞散在分布于成牙本质细胞层，而在冠髓中可见神经再支配。这些免疫活性细胞暂时出现在牙髓-牙本质交界处，表明它们参与了成牙本质细胞的分化以及牙髓再生过程中最初的防御反应。术后第14天，再植牙髓有三种再生模式：(1)修复性牙本质，(2)骨样组织形成，(3)介于两者之间的中间形态。在所有病例中，PAS反应细胞如多形核白细胞(PML)和间充质细胞均见于牙髓间隙。然而，在后两个病例中，炎性细胞的长期停滞也是明显的。因此，PAS反应的结果表明，牙囊来源的细胞向牙髓间隙的迁移和随后适当的牙髓细胞的完全死亡是在再植牙髓中诱导骨样组织形成的决定性因素。
Responses of immunocompetent cells to tooth replantation during the regeneration process of the dental pulp in rat molars were investigated by immunocytochemistry using antibodies to class II major histocompatibility complex (MHC) molecules (OX6 antibody), monocyte/macrophage lineage cells (ED1 antibody) and protein gene product 9.5 (PGP 9.5), as well as by histochemical reaction for periodic acid-Schiff (PAS). Tooth replantation caused an increase in both the number of OX6- and ED1-positive cells and their immunointensity in the replanted pulp, but almost all PGP 9.5-immunoreactive nerves diminished in the initial stages. By postoperative day 3, many OX6- and ED1-immunopositive cells had accumulated along the pulp-dentin border to extend their cytoplasmic processes into the dentinal tubules in successful cases. Once reparative dentin formation had begun after postoperative day 7, OX6- and ED1-immmunopositive cells became scattered in the odontoblast layer, while reinnervation was found in the coronal pulp. The temporal appearance of these immunocompetent cells at the pulp-dentin border suggests their participation in odontoblast differentiation as well as in initial defense reactions during the pulpal regeneration process. On postoperative day 14, the replanted pulp showed three regeneration patterns: (1) reparative dentin, (2) bone-like tissue formation, and (3) an intermediate form between these. In all cases, PAS-reactive cells such as polymorphonuclear leukocytes (PML) and mesenchymal cells occurred in the pulp space. However, the prolonged stagnation of inflammatory cells was also discernible in the latter two cases. Thus, the findings on PAS reaction suggest that the migration of the dental follicle-derived cells into the pulp space and the subsequent total death of the proper pulpal cells are decisive factors for eliciting bone-like tissue formation in the replanted pulp.