Magnetosome-like ferrimagnetic iron oxide nanocubes for highly sensitive MRI of single cells and transplanted pancreatic islets
Magnetosome-like ferrimagnetic iron oxide nanocubes for highly sensitive MRI of single cells and transplanted pancreatic islets
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DOI：
10.1073/pnas.1016409108
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发表时间：
2011-02-15
影响因子：
11.1
通讯作者：
Hyeon, Taeghwan
中科院分区：
文献类型：
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作者：
Lee, Nohyun;Kim, Hyoungsu;Hyeon, Taeghwan
对于超灵敏磁共振成像（MRI），强烈需要具有极高r2弛豫率的磁性纳米颗粒。将聚乙二醇-磷脂（PEG-phospholipid，PEG-phospholipid）包覆于亚铁磁性氧化铁纳米立方体（FIONs）表面，制备了类磁性体纳米颗粒。FION表现出非常高的弛豫率（r2）为324 mM（-1）s（-1），允许有效标记各种细胞。用FION标记的单个细胞的磁共振（MR）成像不仅在体外而且在体内被证明。胰岛移植物及其排斥反应可在1.5T临床MRI扫描仪上使用FION成像。FION的强对比效应使小型啮齿动物和大型动物中移植胰岛的MR成像成为可能。因此，我们期望FIONs用于胰岛移植物的MR成像具有潜在的临床应用价值。此外，FION将在细胞移植后实现高度敏感的非侵入性评估。
For ultrasensitive magnetic resonance imaging (MRI), magnetic nanoparticles with extremely high r2 relaxivity are strongly desired. Magnetosome-like nanoparticles were prepared by coating polyethylene glycol-phospholipid (PEG-phospholipid) onto ferrimagnetic iron oxide nanocubes (FIONs). FIONs exhibited a very high relaxivity (r2) of 324 mM(-1) s(-1), allowing efficient labeling of various kinds of cells. The magnetic resonance (MR) imaging of single cells labeled with FIONs is demonstrated not only in vitro but also in vivo. Pancreatic islet grafts and their rejection could be imaged using FIONs on a 1.5 T clinical MRI scanner. The strong contrast effect of FIONs enabled MR imaging of transplanted islets in small rodents as well as in large animals. Therefore, we expect that MR imaging of pancreatic islet grafts using FIONs has the potentials for clinical applications. Furthermore, FIONs will enable highly sensitive noninvasive assessment after cell transplantation.