A modified method for the in vivo labeling of red blood cells with Tc-99m: concise communication.
A modified method for the in vivo labeling of red blood cells with Tc-99m: concise communication.
复制标题
Tc-99m 体内红细胞标记的改良方法：简明通讯。
DOI：
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发表时间：
1982
影响因子：
9.3
通讯作者：
Strauss Hw
中科院分区：
文献类型：
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作者：
R. Callahan;J. Froelich;K. Mckusick;J. Leppo;Strauss Hw
通过采集血液样本的亚锡DTPA溶液中的血样来测量体内预置的红细胞中~(99)Tc-m的掺入速率，该溶液是~(99)Cs的竞争配体。这种采集技术允许在采样瞬间测量高亲和力的红细胞标记效率。在注射后0.5分钟，只有62%的氚与红细胞紧密结合；在10分钟时，这一比例上升到94.5%。在红细胞分级标记的基础上，改进了体内标记程序，在标记的前10分钟内分离在注射器中灌装的体内高铁酸盐和红细胞。因此，阻止了高铁酸盐分配到血管外隔室，并且在注射时90%的注射的Tc-99m与红细胞牢固地结合。在一系列23名患者中，7名患者接受了体内测试，7名患者接受了改进的体内测试，9名患者分别在不同的场合进行了两种方法的测试。与体内红细胞标记的标准方法相比，改进的方法降低了胃的活动度，改善了图像质量。
The rate of incorporation of Tc-99m into red blood cells pretinned in vivo was measured by collecting blood samples in stannous DTPA solution, which served as a competing ligand for Tc-99m. This collection technique permitted a measurement of high-affinity red-cell labeling efficiency at the instant of sampling. At 0.5 min after injection only 62% of technetium is tightly bound to the red cell; this rises to 94.5% at 10 min. Based on the graded labeling of the red cells, the in vivo labeling procedure was modified by isolating pertechnetate and red blood cells tinned in vivo in a syringe during the first 10 min of labeling. The pertechnetate is thus prevented from distributing to extravascular compartments, and 90% of the injected Tc-99m is firmly bound to red blood cells at the time of injection. In a series of 23 patients, seven were tested with the in vivo method and seven with the modified in vivo method, and nine patients were tested with each method on separate occasions. A decrease in gastric activity and improved image quality were found with the modified method compared with the standard method of in vivo red-cell labeling.