Identification of a five-pass transmembrane protein family localizing in the Golgi apparatus and the ER.
Identification of a five-pass transmembrane protein family localizing in the Golgi apparatus and the ER.
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DOI：
10.1016/j.bbrc.2003.10.197
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发表时间：
2003-12
影响因子：
3.1
通讯作者：
A. Shakoori;G. Fujii;S. Yoshimura;M. Kitamura;K. Nakayama;Takashi Ito;H. Ohno;N. Nakamura
中科院分区：
文献类型：
--
作者：
A. Shakoori;G. Fujii;S. Yoshimura;M. Kitamura;K. Nakayama;Takashi Ito;H. Ohno;N. Nakamura
从蛋白质序列数据库中鉴定了一个五次跨膜蛋白家族（FinGER）。该家族包括酵母Yip 1 p、Yip 4p、Yip 5 p和Yif 1 p，以及它们的植物、昆虫、线虫和哺乳动物同源物，表明它们在广泛的物种中具有保守的功能。在人类中发现了8个家族成员。多重序列比对显示三个区域在所有家族成员中是保守的。所有人类家族成员在各种组织中广泛表达。人类蛋白质定位于高尔基体内及其周围，也可能在一定程度上位于内质网中。高尔基体被五个家族成员的过度表达所破碎。通过酵母双杂交分析，发现其中一些成员相互作用，表明形成了复合物。这些结果表明，FinGERs的功能在维持高尔基体结构和/或ER和高尔基体之间的运输。
A family of five-pass transmembrane proteins (FinGERs) were identified from the protein sequence database. The family includes yeast Yip1p, Yip4p, Yip5p, and Yif1p, and also their plant, insects, nematode, and mammalian homologues, suggesting their conserved function in a broad range of species. Eight family members were found in human. Multiple sequence alignment revealed three regions conserved among all family members. All of the human family members were expressed widely in various tissues. The human proteins were localized in and around the Golgi apparatus and may also be in the ER to some extent. The Golgi apparatus was fragmented by overexpression of the five of the family members. Some of the members were found to interact by yeast two-hybrid analysis, suggesting the formation of a complex. These results suggest that FinGERs function in maintenance of the Golgi structure and/or transport between the ER and the Golgi apparatus.