Influence of receptor activator of nuclear factor (NF)-κB ligand (RANKL), macrophage-colony stimulating factor (M-CSF) and fetal calf serum on human osteoclast formation and activity
Influence of receptor activator of nuclear factor (NF)-κB ligand (RANKL), macrophage-colony stimulating factor (M-CSF) and fetal calf serum on human osteoclast formation and activity
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DOI：
10.1007/s10735-007-9108-3
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发表时间：
2007-08-01
影响因子：
3.2
通讯作者：
Ignatius, Anita
中科院分区：
文献类型：
--
作者：
Kreja, Ludwika;Liedert, Astrid;Ignatius, Anita
用胎牛血清（FCS）刺激6名健康人外周血单个核细胞（PBMNC），在核因子受体激活因子（NF）-κ B配体（RANKL）和巨噬细胞集落刺激因子（M-CSF）的影响下，研究人破骨细胞（OC）的形成和活性。结果表明，在不添加破骨细胞生成因子的情况下，选择性FCS也能刺激OC的形成。通过定量多核抗酒石酸酸性磷酸酶阳性细胞（TRAP+细胞）研究的OC形成以及OC祖细胞对RANKL和M-CSF的敏感性在个体供体之间存在很大差异。OC再吸收活性，在牙本质上的“坑测定”中测量，严格依赖于介质中RANKL和M-CSF的存在，也是供体依赖性的。在研究OC与生物材料的相互作用或细胞因子、生长因子和药物对破骨细胞生成的影响等培养研究中，应考虑供体的巨大差异。
Human osteoclast (OC) formation and activity was studied in cultures of peripheral blood mononuclear cells (PBMNC) from six healthy donors after stimulation with fetal calf serum (FCS), under the influence of the receptor activator of nuclear factor (NF)-kappa B ligand (RANKL) and the macrophage-colony stimulating factor (M-CSF). The results showed that selected FCS could stimulate OC formation without any medium supplementation with osteoclastogenic factors. The OC formation, investigated by quantification of multinucleated tartrate-resistant acid phosphatase-positive cells (TRAP+ cells), and the sensitivity of OC progenitors to RANKL and M-CSF, varied widely between individual donors. The OC resorption activity, measured in the "pit-assay" on dentine, was strictly dependent on the presence of RANKL and M-CSF in the medium and was also donor dependent. The considerable donor variability should be considered in culture studies investigating, e.g. the interactions of OC with biomaterials or the influence of cytokines, growth factors and drugs on osteoclastogenesis.