The intervening sequence excised from the ribosomal RNA precursor of Tetrahymena contains a 5-terminal guanosine residue not encoded by the DNA.
The intervening sequence excised from the ribosomal RNA precursor of Tetrahymena contains a 5-terminal guanosine residue not encoded by the DNA.
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从四膜虫核糖体 RNA 前体中切除的插入序列含有一个不由 DNA 编码的 5 末端鸟苷残基。
DOI：
10.1093/nar/10.9.2823
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发表时间：
1982
影响因子：
14.9
通讯作者：
Cech,TR
中科院分区：
文献类型：
--
作者：
Zaug,AJ;Cech,TR
四膜壳嗜酸菌的核糖体RNA前体含有一个0.4kb的插入序列，该序列被切割成线性RNA分子(“IVS RNA”)，然后环化。对其5‘端标记的IVS RNA进行测序、凝胶分析和末端核苷酸分析。此外，统一标记的IVS RNA被RNAase T1切割，得到的寡核苷酸被二维指纹图谱研究。IVS RNA是一个独特的分子，没有明显的末端异质性。5‘-末端核苷酸是一种鸟苷，由N.Kan和J.Gall确定，它不存在于DNA序列中的相应点(见邻接论文)。该核苷酸在剪接过程中被添加到IVS中[Cech，Zaug和Grabowski(1981)Cell27,487-496]。根据RNA末端附近的序列，RNA序列的其余部分与DNA的序列共线。IVS RNA具有5‘-单磷酸和3’-羟基末端。将这些结果与以前对酵母tRNA干预序列获得的结果进行比较，我们可以得出结论，这两类转录本的剪接机制是根本不同的。
The ribosomal RNA precursor ofTetrahymenathennophilacontains a 0.4 kilobase intervening sequence that is excised as a linear RNA molecule (“IVS RNA”) and subsequently cyclized.Invitrotranscription in isolated nuclei was used to accumulate the IVS RNA. IVS RNA labeled at its 5′ end was subjected to sequencing gel analysis and terminal nucleotide analysis. In addition, uniformly labeled IVS RNA was cleaved with RNAase T1, and the resulting oligo-nucleotides were studied by two-dimensional fingerprinting. The IVS RNA was found to be a unique molecule with no discernible terminal heterogeneity. The 5′-terminal nucleotide is a guanosine that is not present at the corresponding point in the DNA sequence, determined by N. Kan and J. Gall (see adjoining paper). This nucleotide is added to the IVS during splicing [Cech, Zaug, and Grabowski (1981)Cell27, 487–496]. Based on the sequences near the ends of the RNA, the remainder of the RNA sequence is colinear with that of the DNA. The IVS RNA has 5′-monophosphate and 3′-hydroxyl termini. Comparison of these results to those obtained previously for yeast tRNA intervening sequences leads us to conclude that the splicing mechanisms are fundamentally different for these two classes of transcripts.