The non-coding RNA BC1 is down-regulated in the hippocampus of Wistar Audiogenic Rat (WAR) strain after audiogenic kindling
The non-coding RNA BC1 is down-regulated in the hippocampus of Wistar Audiogenic Rat (WAR) strain after audiogenic kindling
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DOI：
10.1016/j.brainres.2010.10.069
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发表时间：
2011-01-07
期刊：
影响因子：
2.9
通讯作者：
Paco-Larson, Maria Luisa
中科院分区：
文献类型：
--
作者：
Goes Gitai, Daniel Leite;Fachin, Ana Lucia;Paco-Larson, Maria Luisa
本研究的目的是确定癫痫易感Wistar听觉大鼠(WAR)听源性癫痫发作的分子通路。为此，我们使用了来自WAR海马区的抑制-消减杂交(SSH)文库，与微阵列比较基因表达分析相结合，随后对单个基因进行了Northern印迹验证。我们发现，与Wistar抗性大鼠以及幼稚Wars和Wistars相比，反复听源性癫痫发作(听源性点燃)的WAR大鼠海马区非蛋白质编码(NPC)RNA Bc1的水平显著降低。通过定量原位杂交，我们证实了听源性点燃动物海马区CA3区Gd-门区Bc1RNA的表达水平较低，放射层和锥体层的信号比显著降低。最近在Bc1-/-小鼠模型中获得的功能结果和我们目前的数据支持Bc1上游信号通路的潜在干扰，与战争的癫痫易感性有关。(C)2010爱思唯尔B.V.保留所有权利。
The aim of this study was to identify molecular pathways involved in audiogenic seizures in the epilepsy-prone Wistar Audiogenic Rat (WAR). For this, we used a suppression-subtractive hybridization (SSH) library from the hippocampus of WARs coupled to microarray comparative gene expression analysis, followed by Northern blot validation of individual genes. We discovered that the levels of the non-protein coding (npc) RNA BC1 were significantly reduced in the hippocampus of WARs submitted to repeated audiogenic seizures (audiogenic kindling) when compared to Wistar resistant rats and to both naive WARs and Wistars. By quantitative in situ hybridization, we verified lower levels of BC1 RNA in the GD-hilus and significant signal ratio reduction in the stratum radiatum and stratum pyramidale of hippocampal CA3 subfield of audiogenic kindled animals. Functional results recently obtained in a BC1-/- mouse model and our current data are supportive of a potential disruption in signaling pathways, upstream of BC1, associated with the seizure susceptibility of WARs. (C) 2010 Elsevier B.V. All rights reserved.