Ethyl alcohol enhances plasminogen activator secretion by endothelial cells.
Ethyl alcohol enhances plasminogen activator secretion by endothelial cells.
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乙醇增强内皮细胞的纤溶酶原激活剂分泌。
DOI：
10.1001/jama.1983.03340060050025
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
W. Laug
中科院分区：
文献类型：
--
作者：
W. Laug
来自大血管的克隆牛内皮细胞融合培养，以恒定的速率产生和分泌纤溶酶原激活物(PA)。将这些培养物暴露在浓度增加的乙醇中，可显著增加PA的产生和分泌。这一效应在孵育3小时后观察到，并在18小时后达到最大，但同时暴露于放线菌胺后，这种效应被消除，这表明活性蛋白质的合成对于观察到的PA活性的增加是必要的。其他脂肪醇如丙醇和丁醇也能显著增加内皮细胞PA的产生和分泌，而已知的乙醇代谢产物乙醛和乙酰辅酶A对细胞的蛋白水解酶分泌没有刺激作用。因此，酒精本身而不是其代谢产物促进了血管内皮细胞分泌PA。这些体外研究可能解释了在适度饮酒的人的血浆中发现的纤溶活性增加的原因。
Confluent cultures of cloned bovine endothelial cells, which were derived from large vessels, produced and secreted plasminogen activator (PA) at constant rates. Exposure of these cultures to increasing concentrations of ethyl alcohol resulted in a significant augmentation of PA production and secretion. This effect, which was observed after three hours and was maximal after 18 hours of incubation, was abolished by simultaneous exposure of the cultures to cycloheximide, indicating that active protein synthesis was necessary for the observed increase of PA activity. Other aliphatic alcohols such as propanol and butanol also significantly augmented PA production and secretion by endothelial cells, whereas acetaldehyde and acetylcoenzyme A, known metabolites of ethyl alcohol, had no stimulatory effect on protease secretion by the cells. Therefore, the alcohol itself rather than its metabolites was responsible for the enhancement of PA secretion by endothelial cells. These in vitro studies may explain the increased fibrinolytic activity found in the plasma of persons consuming moderate amounts of alcohol.