Bioabsorbable device to prepare subcutaneous pockets for islet transplantation
Bioabsorbable device to prepare subcutaneous pockets for islet transplantation
复制标题
为胰岛移植准备皮下袋的生物可吸收装置
DOI：
10.1002/jbm.b.34203
复制
发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Iwata Hiroo
中科院分区：
文献类型：
--
作者：
Kuwabara Rei;Iwata Hiroo
研究表明，微创胰岛移植和长期维持胰岛移植物而不使用免疫抑制剂可用于治疗1型糖尿病。在临床上，使用由该材料制成的装置制备围绕血管丰富组织的皮下囊袋用于胰岛移植。在这里，明胶片被植入到两个背部皮下网站在糖尿病ACI大鼠，和bFGF和透明质酸钠溶液的混合物被注射到周围的明胶片。在注射bFGF混合物7天后，将从F344大鼠分离的总共1500个胰岛移植到每个袋中。10只接受同种异体胰岛移植的糖尿病ACI大鼠中有9只在未给予免疫抑制剂的情况下表现出长期的正常反应。植入后67天，明胶片几乎消失。因此，可以使用明胶片和透明质酸钠-bFGF混合物制备皮下免疫耐受部位。移植到该部位的同种异体胰岛可以存活并长期控制血糖水平，即使没有免疫抑制。© 2018 Wiley Periodicals，Inc. J Biomed Mater Res Part B：Appl Biomater 107 B：1107-1112，2019。
Small invasive transplantation of islets and long maintenance of the islet graft without immunosuppression has been studied for the treatment of type 1 diabetes. Clinically, subcutaneous pockets surrounding vascular‐rich tissue are prepared for islet transplantation using a device made of the materials. Here, gelatin sheets were implanted into two dorsal subcutaneous sites in diabetic ACI rats, and a mixture of bFGF and sodium hyaluronate solution was injected around the gelatin sheets. A total of 1500 islets isolated from F344 rats were transplanted into each of the pockets 7 days after injection of the bFGF mixture. Nine of 10 diabetic ACI rats with allogeneic islet graft demonstrated long‐term normoglycemia without administration of immunosuppressant. Gelatin sheets almost disappeared 67 days after implantation. Thus, subcutaneous immune‐tolerant sites can be prepared using gelatin sheets and a sodium hyaluronate–bFGF mixture. Allogeneic islets transplanted into the sites can survive and control blood glucose levels for a long period, even without immunosuppression. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1107–1112, 2019.