ISOLATION OF ALCOHOL OXIDASE AND 2 OTHER METHANOL REGULATABLE GENES FROM THE YEAST PICHIA-PASTORIS
ISOLATION OF ALCOHOL OXIDASE AND 2 OTHER METHANOL REGULATABLE GENES FROM THE YEAST PICHIA-PASTORIS
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DOI：
10.1128/mcb.5.5.1111
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发表时间：
1985-01-01
影响因子：
5.3
通讯作者：
GINGERAS, TR
中科院分区：
文献类型：
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作者：
ELLIS, SB;BRUST, PF;GINGERAS, TR
甲醇的氧化遵循明确的途径，并且对于几种甲基营养型酵母是相似的。使用甲醇作为用于巴斯德毕赤酵母生长的唯一C源刺激基因家族的表达。三个甲醇响应基因被分离出来; c [互补] DNA拷贝从这些基因的mRNA，并从体外翻译的蛋白质产物进行了检查。通过酶、免疫学和序列分析，确定醇氧化酶为克隆的甲醇调控基因之一。这3个分离基因中的每一个的甲醇调节表达都发生在转录水平。从巴斯德毕赤酵母中分离出2个甲醇响应性基因组克隆的DNA亚片段，并初步鉴定为含有参与甲醇调节的控制区。
The oxidation of methanol follows a well-defined pathway and is similar for several methylotrophic yeasts. The use of methanol as the sole C source for the growth of P. pastoris stimulates the expression of a family of genes. Three methanol-responsive genes were isolated; c [complementary] DNA copies were made from mRNA of these genes, and the protein products from in vitro translations were examined. The identification of alcohol oxidase as one of the cloned, methanol-regulated genes was made by enzymatic, immunological and sequence analyses. Methanol-regulated expression of each of these 3 isolated genes occurs at the level of transcription. DNA subfragments of 2 of the methanol-responsive genomic clones from P. pastoris were isolated and tentatively identified as containing the control regions involved in methanol regulation.