Novel microRNAs encoded by duck enteritis virus.
Novel microRNAs encoded by duck enteritis virus.
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DOI：
10.1099/vir.0.040634-0
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发表时间：
2012-07
期刊：
影响因子：
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通讯作者：
Yongxiu Yao;Lorraine P. Smith;L. Petherbridge;M. Watson;V. Nair
中科院分区：
文献类型：
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作者：
Yongxiu Yao;Lorraine P. Smith;L. Petherbridge;M. Watson;V. Nair
鸭肠炎病毒(DEV)是一种重要的疱疹病毒病原，与水禽的急性、高度接触性致死性疾病有关。利用对感染鸡胚胎成纤维细胞培养物中的RNA进行深度测序的方法，我们鉴定了几个新的DEV编码的微(Mi)RNA。与大多数有脊椎动物编码的miRNAs不同，DEV编码的miRNAs主要映射到基因组的独特长区。DEV miR-D18和miR-D19的前体相互重叠，表明与miRNA偏移RNA相似，尽管在报告分析中只有DEV-miR-D18-3p具有功能。识别这些新的miRNAs将增加越来越多的病毒编码的miRNAs，从而能够探索它们在发病机制中的作用。
Duck enteritis virus (DEV) is an important herpesvirus pathogen associated with acute, highly contagious lethal disease in waterfowls. Using a deep sequencing approach on RNA from infected chicken embryo fibroblast cultures, we identified several novel DEV-encoded micro (mi)RNAs. Unlike most mardivirus-encoded miRNAs, DEV-encoded miRNAs mapped mostly to the unique long region of the genome. The precursors of DEV miR-D18 and miR-D19 overlapped with each other, suggesting similarities to miRNA-offset RNAs, although only the DEV-miR-D18-3p was functional in reporter assays. Identification of these novel miRNAs will add to the growing list of virus-encoded miRNAs enabling the exploration of their roles in pathogenesis.