Prediction Value of Cyclin Ds in Cisplatin Sensitivity of Oral and Maxillofacial Squamouse Cell Carcinoma
Prediction Value of Cyclin Ds in Cisplatin Sensitivity of Oral and Maxillofacial Squamouse Cell Carcinoma
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Cyclin Ds对口腔颌面部鳞状细胞癌顺铂敏感性的预测价值
DOI：
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发表时间：
2005
期刊：
影响因子：
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通讯作者：
Qiu Wei
中科院分区：
文献类型：
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作者：
Qiu Wei
目的：筛选并鉴定口腔鳞癌顺铂敏感性相关基因及其表达谱，有助于阐明口腔鳞癌耐药的原因，指导临床化疗。研究方法：收集口腔鳞癌细胞样本，根据顺铂体外改良MTT法的结果分为两组。采用免疫组化法检测CCND 1、CCND 3、GST-Pi和P-gp的表达情况。结果如下：根据改良MTT法的结果，将样本分别分为顺铂耐药组（23例）和顺铂敏感组（20例）。CCND 1和CCND 3在两组间的阳性率差异有统计学意义，而GST-Pi和P-gp在两组间的阳性率差异无统计学意义。结论：CCND 1和CCND 3的表达状态可作为顺铂耐药相关基因表达谱的一个组成部分.
Objective: Screen and identify those cisplatin-sensitivity related genes and expression profiles in oral squamous cell carcinoma, to help illustrate the underlined reason of drug resistant and direct the chemotherapy in clinic. Methods: Oral squamouse cell samples were collected and divided into two groups according to the results of in vitro modified MTT assay with cisplatin. Immunohistochemical tests were carried out to test the expression status of CCND1, CCND3,GST-Pi and P-gp. Results: According to results of modified MTT assay, the samples were clustered into cisplatin resistant groups(23 Samples) and cisplatin sensitive groups(20 Samples) respectively. There were significant difference in positive rate of CCND1 and CCND3 between these two groups, but not in GST-Pi and P-gp. Conclusion: Expression status of CCND1 and CCND3 could be included in cisplatin -resistant related gene expression profile.