Dendritic cells phagocytose and are activated by Treponema pallidum
Dendritic cells phagocytose and are activated by Treponema pallidum
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DOI：
10.1128/iai.69.1.518-528.2001
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发表时间：
2001-01-01
影响因子：
3.1
通讯作者：
Norgard, MV
中科院分区：
文献类型：
--
作者：
Bouis, DA;Popova, TG;Norgard, MV
梅毒是一种由螺旋体细菌梅毒螺旋体引起的人类性传播疾病，细胞介导的免疫过程在梅毒的临床表现中起着突出作用。然而，树突状细胞（dc）是第一个在皮肤或粘膜（早期梅毒感染的主要部位）内遇到抗原的免疫能力细胞，而皮肤或粘膜是早期梅毒感染的主要部位。在本研究中，利用来自小鼠皮肤的未成熟DC系XS52，研究了苍白球绦虫与DC的相互作用和随后的DC激活（成熟）。电镜显示苍白球通过螺旋吞噬和常规吞噬被树突状细胞吞噬，并被输送到膜结合液泡中。XS52 DC细胞系表达表面CD14和toll样受体2和4的mRNA，这些分子包含细菌调节素激活免疫细胞的重要信号成分。T. pallidum和一种合成的脂肽（对应于47-kDa的主要膜脂蛋白）激活了XS52 DC细胞系，这可以通过白细胞介素-12 （IL-12）、IL-1 β、肿瘤坏死因子α和IL-6的分泌以及CD54表面表达的升高来证明。综合数据支持梅毒t和/或其促炎膜脂蛋白刺激的dc参与驱动典型梅毒的细胞免疫过程的论点。
Cell-mediated immune processes play a prominent role in the clinical manifestations of syphilis, a sexually transmitted disease of humans caused by spirochetal bacterium Treponema pallidum. The immune cell type that initiates the early immune response to T. pallidum thus far has not been identified, However, dendritic cells (DCs) are the first immune-competent cells to encounter antigens within skin or mucous membranes, the principal sites of early syphilitic infection. In the present study, immature DC line XS52, derived from murine skin, was utilized to examine T. pallidum-DC interactions and subsequent DC activation (maturation). Electron microscopy revealed that T. pallidum was engulfed by DCs via both coiling and conventional phagocytosis and was delivered to membrane-bound vacuoles. The XS52 DC line expressed surface CD14 and mRNA for Toll-like receptors 2 and 4, molecules comprising important signaling components for immune cell activation by bacterial modulins. Both T. pallidum and a synthetic lipopeptide (corresponding to the 47-kDa major membrane lipoprotein) activated the XS52 DC line, as indicated by the secretion of interleukin-12 (IL-12), IL-1 beta, tumor necrosis factor alpha, and IL-6 and elevated surface expression of CD54. The combined data support the contention that DCs stimulated by T. pallidum and/or its proinflammatory membrane lipoproteins are involved in driving the cellular immune processes that typify syphilis.