The pigment epithelium-derived factor (PEDF): an important potential therapeutic agent for infantile hemangioma
The pigment epithelium-derived factor (PEDF): an important potential therapeutic agent for infantile hemangioma
复制标题
DOI：
10.1007/s00403-017-1716-5
复制
发表时间：
2017-02
影响因子：
3
通讯作者：
Ming Li;Yanru Chen;Zhihui Guo;Yi-de Xie;Ya-kuan Zhou;Cheng-Hong Jiang;Xiaosong Chen
中科院分区：
文献类型：
--
作者：
Ming Li;Yanru Chen;Zhihui Guo;Yi-de Xie;Ya-kuan Zhou;Cheng-Hong Jiang;Xiaosong Chen
在以往的研究中，促血管生成因子在婴儿血管瘤中的表达和作用已有很好的研究。然而，血管生成抑制剂的作用很少被揭示。PEDF作为一种最强、最安全的内源性抑制物，其表达情况目前尚不清楚。为探讨色素上皮衍生因子(PEDF)在婴幼儿血管瘤增生期和退化期的表达及意义，采用免疫组织化学方法检测了42例增生期、40例退化期和11例未消退期先天性血管瘤组织中PEDF、血管内皮生长因子、Ki-67和CD34蛋白的表达。同时，用实时定量RT-PCR方法检测这些因子的mRNA表达。退化期PEDF的蛋白和基因表达显著高于增生期和NICH(P<； 0.001)，而VEGFmRNA和蛋白的表达明显低于增生期和NICH(P<； 0.001)。微血管密度(MVD)、Ki-67的变化与PEDF、VEGF的表达呈正相关。这些结果表明，血管内皮生长因子的减少和PEDF的增加是婴幼儿血管瘤发生发展的原因。PEDF可能在婴幼儿血管瘤的自发消退中起关键作用，有望成为婴幼儿血管瘤的一种重要的潜在治疗药物。
In previous studies, the expression and the role of proangiogenic factors in infantile hemangiomas have been well studied. However, the role of angiogenic inhibitors has been revealed rarely. The expression of PEDF, as the strongest and safe endogenous inhibitor, is still unrecognized until the current study. In order to investigate the expression and significance of the pigment epithelium-derived factor (PEDF) in the proliferating and regressing phases of infantile hemangiomas, the expression of PEDF, VEGF, Ki-67, and CD34 protein in hemangioma tissues was examined with immunohistochemical polymer HRP method in 42 cases during the proliferative phase, 40 cases during the regressing phase, and 11 cases of non-involuting congenital hemangiomas (NICHs). Meanwhile, the mRNA expression of these factors was detected with quantitative realtime RT-PCR. We found the protein and mRNA expression of PEDF in regressing phase was significantly higher than those in proliferative phase and NICHs (P< 0.001), while the protein and mRNA expression of VEGF were much lower (P< 0.001). The microvessel density (MVD), Ki-67 changes, and the expression of PEDF and VEGF were found significantly correlated. These results indicated that the reduction of VEGF and increase in PEDF are causative to the evolution of infantile hemangioma. PEDF may play a key role in the spontaneous regression of infantile hemangioma and may become an important potential therapeutic agent for infantile hemangioma.