Absorption, metabolism and effect of compatibility on absorption of qishenyiqi dropping pill
Absorption, metabolism and effect of compatibility on absorption of qishenyiqi dropping pill
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芪参益气滴丸的吸收、代谢及配伍对吸收的影响
DOI：
10.1002/bmc.3069
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发表时间：
2014-04-01
影响因子：
1.8
通讯作者：
Luo, Guo-an
中科院分区：
文献类型：
--
作者：
Han, Yan-qi;Wang, Jing;Luo, Guo-an
气血益气滴丸（QSYQ）是中国治疗心脏病的传统中药处方。关于QSYQ活性化合物和代谢成分的系统鉴定知识普遍缺乏。因此，建立一种有效的复方有效成分分析方法和中药相互作用测定方法是十分必要的。采用计算化学预测、改进的体外肠囊实验、Caco-2细胞单层体外实验、大鼠体内实验和超高效液相色谱/二极管阵列检测/四极杆飞行时间质谱（UPLC/DAD/Q-TOF MS）分析了QSYQ的可吸收化合物和代谢产物。通过UPLC/DAD/Q-TOF ms鉴定出8种代谢物。配伍后，酚酸和皂苷的吸收率显著提高，异黄酮的吸收受到抑制。挥发油成分对其他化合物的吸收有促进作用，而对其自身的吸收有抑制作用。综上所述，本研究建立了一种快速有效的方法来展示芪syq的吸收代谢和揭示草药间的配型关系。本研究可为方剂配伍及中药现代化建设提供参考。版权所有：John Wiley & Sons， Ltd。
Qishenyiqi dropping pill (QSYQ), is a traditional Chinese medicine (TCM) prescription for treating heart diseases in China. Knowledge concerning the systemic identification of active compounds and metabolic components of QSYQ is generally lacking. Therefore, it is essential to develop a valid method for the analysis of active compounds of the combined prescription and determination of interactions among the herbs. The absorbable compounds and metabolites of QSYQ were profiled using computational chemistry prediction, an improved everted gut sac in vitro experiment, the Caco-2 cell monolayer in vitro test, a rat in vivo experiment and ultra-performance liquid chromatography/diode array detection/quadrupole-time of flight mass spectrum (UPLC/DAD/Q-TOF MS). In total, 42 prototype compounds were recognized as absorbable compounds, and eight metabolites were identified by UPLC/DAD/Q-TOF MS. The absorption rates of phenolic acids and saponins were significantly improved and the absorption of isoflavone was inhibited after compatibility. The volatile oil component had an improved effect on the absorption of other compounds, while its own absorption was inhibited. In conclusion, the present study established a rapid and effective strategy for demonstrating the absorption and metabolism of QSYQ and revealing the compatible relationship among herbs. This investigation can provide a reference for the compatibility of prescriptions and the modernization of TCM. Copyright (c) 2013 John Wiley & Sons, Ltd.