Expression of a salt-induced protein (SALT) in suspension-cultured cells and leaves of rice following exposure to fungal elicitor and phytohormones
Expression of a salt-induced protein (SALT) in suspension-cultured cells and leaves of rice following exposure to fungal elicitor and phytohormones
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DOI：
10.1007/s00299-004-0836-5
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发表时间：
2004-10-01
影响因子：
6.2
通讯作者：
Kang, KY
中科院分区：
文献类型：
--
作者：
Kim, ST;Kim, SG;Kang, KY
植物激素是调节胁迫和防御相关基因的重要信号化合物。然而，没有明确的证据表明这些信号分子和生物激发子对悬浮培养的水稻细胞中的SALT基因的调控有任何影响。我们的特点是一个盐基因的表达与真菌诱导子，植物激素，放线菌酮，和蛋白激酶/磷酸酶抑制剂治疗后。用真菌诱导子、茉莉酸（JA）、脱落酸（阿坝）和NaCl处理后，SALT表达上调。然而，水杨酸（SA）单独或与其他激发子之一，不仅强烈抑制SALT基因的表达，但也表现出拮抗作用的悬浮细胞和叶片。放线菌酮抑制悬浮细胞和叶片中盐的积累，但蛋白激酶/磷酸酶抑制剂没有。免疫组织化学分析表明，SALT蛋白主要分布于大、小叶脉维管束的木质部薄壁细胞中。
Phytohormones are essential signal compounds in the regulation of stress-related and defense-related genes. However, there is no clear evidence for any effect of these signal molecules and biotic elicitors on the regulation of the SALT gene in suspension-cultured rice cells. We characterized the expression of a SALT gene following treatment with fungal elicitor, phytohormones, cycloheximide, and inhibitors of protein kinase/phosphatases. SALT expression was up-regulated following treatment with a fungal elicitor, jasmonic acid (JA), abscisic acid (ABA), and NaCl. However, salicylic acid (SA) alone or in combination with one of the other elicitors not only strongly inhibited SALT gene expression but also exhibited an antagonistic effect in suspension cells and leaves. Cycloheximide inhibited SALT accumulation in suspension cells and in leaves, but the inhibitors of protein kinase/phosphatase did not. Immunolocalization revealed that SALT protein was present in xylem parenchyma cells of vascular bundles in the major and minor leaf veins.