Soggy, a spermatocyte-specific gene, lies 3.8 kb upstream of and antipodal to TEAD-2, a transcription factor expressed at the beginning of mouse development
Soggy, a spermatocyte-specific gene, lies 3.8 kb upstream of and antipodal to TEAD-2, a transcription factor expressed at the beginning of mouse development
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DOI：
10.1093/nar/28.20.3982
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发表时间：
2000-10-15
影响因子：
14.9
通讯作者：
DePamphilis, ML
中科院分区：
文献类型：
--
作者：
Kaneko, KJ;DePamphilis, ML
通过对小鼠着床前发育早期表达的mTEAD-2基因调控区的研究，我们发现在mTEAD-2基因上游3.8kb处发现了一个新的基因，该基因是一个单拷贝的睾丸特异性基因，称为Soggy（mSgy），它产生一个长度约为1.3kb的显性mRNA。它以与mTEAD-2相反的方向转录，因此将这两个基因的调控元件紧密靠近。mSgy含有三个可能作为翻译起始位点的甲硫氨酸密码子，但大多数mSGY蛋白体外合成是从第一个Met密码子开始以产生全长蛋白，这表明mSGY通常由230个氨基酸组成（26.7 kDa），转录开始于与第一个Met密码子上游150 bp相似的核苷酸簇，使用TATA-上游前0.9kb内含有较少的启动子。在不表达mSgy的细胞中，该启动子的活性受到上游序列-0.9和-2.5 kb之间的抑制，但在表达mSgy的细胞中这种抑制被解除，mSgy mRNA仅在胚胎中检测到，并且仅在生精小管的发育精母细胞中检测到，表明mSgy是精母细胞特异性基因。由于mTEAD-2和mSgy在相同细胞中不表达，因此mSgy/mTEAD-2焦点为哺乳动物发育期间基因表达的差异调节提供了独特的范例。
Investigation of the regulatory region of mTEAD-2, a gene expressed at the beginning of mouse preimplantation development, led to the surprising discovery of another gene only 3.8 kb upstream of mTEAD-2, Here we show that this new gene is a single copy, testis-specific gene called Soggy (mSgy) that produces a single, dominant mRNA similar to1.3 kb in length. It is transcribed in the direction opposite to mTEAD-2, thus placing the regulatory elements of these two genes in close proximity. mSgy contains three methionine codons that could potentially act as translation start sites, but most mSGY protein synthesis in vitro was initiated from the first Met codon to produce a full-length protein, suggesting that mSGY normally consists of 230 amino acids (26.7 kDa), Transcription began at a cluster of nucleotides similar to 150 bp upstream of the first Met codon using a TATA-less promoter contained within the first 0.9 kb upstream. The activity of this promoter was repressed by upstream sequences between -0.9 and -2.5 kb in cells that did not express mSgy, but this repression was relieved in cells that did express mSgy, mSgy mRNA was detected in embryos only after day 15 and in adult tissues only in the developing spermatocytes of seminiferous tubules, suggesting that mSgy is a spermatocyte-specific gene. Since mTEAD-2 and mSgy were not expressed in the same cells, the mSgy/mTEAD-2 focus provides a unique paradigm for differential regulation of gene expression during mammalian development.