Visualization, characterization, and turnover of CD8+ memory T cells in virus-infected hosts.
Visualization, characterization, and turnover of CD8+ memory T cells in virus-infected hosts.
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DOI：
10.1084/jem.183.4.1367
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发表时间：
1996-04-01
期刊：
影响因子：
--
通讯作者：
Pircher H
中科院分区：
文献类型：
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作者：
Zimmerman C;Brduscha-Riem K;Blaser C;Zinkernagel RM;Pircher H
T细胞记忆的细胞基础是一个有争议的问题，并且由于无法在体内诱导和追踪对特定抗原具有特异性的长期记忆T细胞，阻碍了进展。通过使用淋巴细胞性脉络丛脑膜炎病毒 (LCMV) 感染的小鼠模型和采用来自表达 LCMV 特异性 T 细胞受体的转基因小鼠的 CD8+ T 细胞的过继转移系统，生成了一组对 LCMV 具有特异性的真实记忆 T 细胞，并进行了体内分析。在感染 LCMV 的 C57BL/6 受体小鼠中，转基因 T 细胞先扩增（1,000 倍），然后减少（10 倍），表现出以下特征：(a) 平均细胞大小比初始小鼠大，但小于第 8 天的效应细胞； (b) 细胞表面“记忆”标记表达的异质性； (c) 当从受体脾脏中分离时直接溶细胞。通过体内溴脱氧尿苷标记实验，分析了受者体内这些 LCMV 特异性记忆 T 细胞的时间依赖性增殖活性。实验表明，在没有细胞分裂的情况下，LCMV 特异性 CD8+ 记忆 T 细胞可以在 LCMV 免疫小鼠中持续存在较长时间（>2 个月）；记忆群体作为一个整体存活了11个月以上。
The cellular basis of T cell memory is a controversial issue and progress has been hampered by the inability to induce and to trace long- term memory T cells specific for a defined antigen in vivo. By using the murine model of lymphocytic choriomeningitis virus (LCMV) infection and an adoptive transfer system with CD8+ T cells from transgenic mice expressing an LCMV-specific T cell receptor, a population of authentic memory T cells specific for LCMV was generated and analyzed in vivo. The transgenic T cells that have expanded (1,000-fold) and then decreased (10-fold) in LCMV-infected C57BL/6 recipient mice exhibited the following characteristics: they were (a) of larger average cell size than their naive counterparts but smaller than day 8 effector cells; (b) heterogeneous with respect to expression of cell surface "memory" markers; and (c) directly cytolytic when isolated from recipient spleens. The time-dependent proliferative activity of these LCMV-specific memory T cells was analyzed in the recipients by bromodeoxyuridine labeling experiments in vivo. The experiments revealed that LCMV-specific CD8+ memory T cells can persist in LCMV- immune mice for extended periods of time (>2 mo) in the absence of cell division; the memory population as a whole survived beyond 11 mo.