Ultrastructural localization of the core protein of a basement membrane-specific chondroitin sulfate proteoglycan in adult rat skin.
Ultrastructural localization of the core protein of a basement membrane-specific chondroitin sulfate proteoglycan in adult rat skin.
复制标题
成年大鼠皮肤中基底膜特异性硫酸软骨素蛋白多糖核心蛋白的超微结构定位。
DOI：
10.1007/bf00372091
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发表时间：
1990
影响因子：
3
通讯作者：
Fine,JD
中科院分区：
文献类型：
--
作者：
McCarthy,KJ;Horiguchi,Y;Couchman,JR;Fine,JD
基底膜是存在于组织的上皮/间充质界面处的复杂细胞外基质。真皮-表皮连接处已显示含有许多组分，其中一些最熟知的是层粘连蛋白、IV型和VII型胶原、硫酸乙酰肝素蛋白聚糖、纤连蛋白和巢蛋白/巢蛋白。在本文中，我们显示，使用核心蛋白特异性抗体，存在一个新描述的基底膜特异性硫酸软骨素蛋白多糖在上皮/间充质界面的成年大鼠皮肤。在超微结构上，这种抗原被证明主要存在于基底层内，显然集中在致密层中。此外，一些蛋白多糖也存在于致密层下方，与网状层胶原纤维相关。
Basement membranes are complex extracellular matrices present at epithelial/mesenchymal interfaces of tissues. The dermal-epidermal junction has been shown to contain numerous components, some of the most well known being laminin, types IV and VII collagens, heparan sulfate proteoglycan, fibronectin, and entactin/nidogen. In this paper we show, using core protein-specific antibodies, the presence of a newly described basement membrane-specific chondroitin sulfate proteoglycan at the epithelial/ mesenchymal interface of adult rat skin. Ultrastructurally, this antigen was proven to reside primarily within the basal lamina, apparently concentrated in the lamina densa. In addition, some of the proteoglycan was also present beneath the lamina densa, associated with the reticular lamina collagen fibrils.