Preparation and characterization of molecularly imprinted organic–inorganic hybrid materials by sol–gel processing for selective recognition of ibuprofen
Preparation and characterization of molecularly imprinted organic–inorganic hybrid materials by sol–gel processing for selective recognition of ibuprofen
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DOI：
10.1007/s10971-013-2966-0
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发表时间：
2013-01
影响因子：
2.5
通讯作者：
Ling Zhou;Yanyue Kong;Shian Zhong;Xiaorun Zhou
中科院分区：
文献类型：
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作者：
Ling Zhou;Yanyue Kong;Shian Zhong;Xiaorun Zhou
采用半共价印迹法，以非甾体抗炎药布洛芬为模板，通过溶胶-凝胶法制备分子印迹聚合物（MIP）。首次采用多步合成法合成了一种功能单体，并将其用于合成分子印迹复合物。用傅里叶变换红外光谱和氮气吸附对聚合物进行了表征。采用薄层层析法评价MIP的特异性分子识别能力。此外，对分子印迹聚合物印迹布洛芬的动力学和热力学进行了研究。平衡重结合实验结果表明，分子印迹聚合物对布洛芬具有良好的吸附性能。Scatchard分析表明，模板-聚合物系统仅显示单位点结合行为，解离常数为1.84 mmol L−1。动态吸附呈准二级动力学。ΔHθ为正值，ΔGθ为负值，说明MIP结合系统是吸热的、自发的。
This work adopted semi-covalent imprinting to prepare molecularly imprinted polymers (MIP) with ibuprofen, a non-steroidal anti-inflammatory drug, as template by sol–gel processing, which is characterized by both the high affinity of covalent binding and the mild operation conditions of non-covalent rebinding. A functional monomer, which was used to synthesize the monomer-imprinted molecule complex, was prepared by multi-step synthesis for the first time. MIP was characterized by Fourier transform IR spectrum and nitrogen adsorption. Thin-layer chromatography separation was used to evaluate the specific molecular recognition ability of MIP. In addition, dynamic and thermodynamic studies on MIP imprinting ibuprofen were undertaken. The results of equilibrium rebinding experiments showed that MIP exhibited good adsorption capacity for ibuprofen. Scatchard analysis illustrated that the template-polymer system shows only one-site binding behavior with a dissociation constant of 1.84 mmol L−1. Dynamic adsorption exhibited pseudo-second-order kinetics. The positive value of ΔHθand the negative values of ΔGθdemonstrated that the binding system for MIP is endothermic and spontaneous.