INHIBITION OF CELL-CYCLE PROGRESSION BY ANTISENSE OLIGODEOXYNUCLEOTIDES
INHIBITION OF CELL-CYCLE PROGRESSION BY ANTISENSE OLIGODEOXYNUCLEOTIDES
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DOI：
10.1111/j.1749-6632.1992.tb21058.x
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发表时间：
1992-10-28
期刊：
影响因子：
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通讯作者：
SURMACZ, E
中科院分区：
文献类型：
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作者：
BASERGA, R;REISS, K;SURMACZ, E
我们已经使用反义策略来研究某些基因在细胞周期进程中的作用。特别地，我们使用反义寡脱氧核苷酸来研究：（1）IGF-1受体在控制细胞增殖中的作用;和（2）细胞周期中基因表达的顺序。我们的研究结果可以概括如下：（1）IGF-1受体被其配体IGF-1激活是成纤维细胞和造血细胞增殖的必要步骤;（2）DNA合成基因的表达，如PCNA，DNA聚合酶α和cdc2，依赖于先前基因的表达。初步的时间顺序为：c-myc> c-myb> IGF-1受体> DNA合成基因。
We have used the antisense strategy to study the role of certain genes in cell cycle progression. In particular, we used antisense oligodeoxynucleotides to study:(1) the role of the IGF-1 receptor in the control of cell proliferation; and (2) the sequence of gene expression during the cell cycle. Our results can be summarized as follows:(1) the activation of the IGF-1 receptor by its ligand, IGF-1, is an obligatory step in the proliferation of fibroblasts and hemopoietic cells; and (2) the expression of DNA synthesis genes, such as PCNA, DNA polymerase alpha, and cdc2, is dependent on the expression of previous genes. A tentative temporal order is: c-myc> c-myb> IGF-1 receptor> DNA synthesis genes.