African water storage pots for the delivery of the entomopathogenic fungus Metarhizium anisopliae to the malaria vectors Anopheles gambiae s.s. and Anopheles funestus
African water storage pots for the delivery of the entomopathogenic fungus Metarhizium anisopliae to the malaria vectors Anopheles gambiae s.s. and Anopheles funestus
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DOI：
10.4269/ajtmh.2008.78.910
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发表时间：
2008-06-01
影响因子：
3.3
通讯作者：
Knols, Bart G. J.
中科院分区：
文献类型：
--
作者：
Farenhorst, Marit;Farina, Daniela;Knols, Bart G. J.
我们研究了使用非洲储水罐点源应用金龟子绿僵菌对疟疾媒介冈比亚按蚊s.s.和一个. funestus。粘土罐被证明是有吸引力的休息场所，男性和女性安。冈比亚公司并且在用真菌浸渍后不具有驱避性。M.绿僵菌在盆内喷洒后具有高度感染性和毒性。在4 × 1010分生孢子/m2的剂量下，平均95 +/- 1.2%的An.冈比亚公司真菌感染1 × 10（10）分生孢子/m2的较低剂量平均感染91.5 +/- 0.6%的An。冈比亚公司和91.8 ± 1.2%的An. funestus蚊子真菌感染显着减少蚊子寿命，如存活曲线和LT，值之间的差异所示。这些盆是适合应用昆虫病原真菌对疟疾病媒和可持续的现场实施的潜力进行了讨论。
We studied the use of African water storage pots for point source application of Metarhizium anisopliae against the malaria vectors Anopheles gambiae s.s. and An. funestus. Clay pots were shown to be attractive resting sites for male and female An. gambiae s.s. and were not repellent after impregnation with fungus. M. anisopliae was highly infective and virulent after spray application inside pots. At a dosage of 4 x 10(10) conidia/m(2), an average of 95 +/- 1.2% of An. gambiae s.s. obtained a fungal infection. A lower dosage of 1 x 10(10) conidia/m(2) infected an average of 91.5 +/- 0.6% of An. gambiae s.s. and 91.8 +/- 1.2% of An. funestus mosquitoes. Fungal infection significantly reduced mosquito longevity, as shown by differences between survival curves and LT,, values. These pots are suitable for application of entomopathogenic fungi against malaria vectors and their potential for sustainable field implementation is discussed.