Selenoprotein W is a glutathione-dependent antioxidant in vivo
Selenoprotein W is a glutathione-dependent antioxidant in vivo
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DOI：
10.1016/s0014-5793(02)02628-5
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发表时间：
2002-04-24
期刊：
影响因子：
3.5
通讯作者：
Kim, IY
中科院分区：
文献类型：
--
作者：
Jeong, DW;Kim, TS;Kim, IY
从小鼠脑组织中克隆了硒蛋白W（Se-W）的cDNA，并在CHO细胞和H1299人肺癌细胞中表达，研究了Se-W的功能。Se-W的过表达显著降低了两种细胞系对H2 O2细胞毒性的敏感性。转染细胞的细胞内过氧化物浓度低于亲本细胞在细胞外H2 O2的存在或不存在下。硒-W对氧化应激的抗性依赖于谷胱甘肽。硒-W突变体的表达，其中硒代半胱氨酸-13或半胱氨酸-37被丝氨酸取代，并没有赋予14202的抗性，暗示这些残基在体内的硒-W的抗氧化活性。(C)2002年欧洲生物化学学会联合会。由Elsevier Science B. V.出版，版权所有。
The function of selenoprotein W (Se-W) was investigated by cloning the corresponding cDNA from mouse brain and expressing it in CHO cells and H1299 human lung cancer cells. Overexpression of Se-W markedly reduced the sensitivity of both cell lines to H2O2 cytotoxicity. The intracellular peroxide concentration of the transfected cells was lower than that of the parental cells in the absence or presence of extracellular H2O2. The resistance to oxidative stress conferred by Se-W was dependent on glutathione. Expression of Se-W mutants in which selenocysteine-13 or cysteine-37 was replaced by serine did not confer resistance to 14202, implicating these residues in the antioxidant activity of Se-W in vivo. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.