Expression and prognostic role of MMP2, MMP9, MMP13, and MMP14 matrix metalloproteinases in sinonasal and oral malignant melanomas
Expression and prognostic role of MMP2, MMP9, MMP13, and MMP14 matrix metalloproteinases in sinonasal and oral malignant melanomas
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DOI：
10.1016/j.humpath.2007.07.003
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发表时间：
2008-03-01
期刊：
影响因子：
3.3
通讯作者：
Laver, Nora V.
中科院分区：
文献类型：
--
作者：
Kondratiev, Svetlana;Gnepp, Douglas R.;Laver, Nora V.
鼻腔和口腔恶性黑色素瘤是罕见的恶性肿瘤，占所有黑色素瘤的比例不到2%。基质金属蛋白酶（MMP）是多种生理和病理过程（包括伤口愈合、胚胎发生、肿瘤侵袭和转移）中细胞外基质降解所需的蛋白水解酶。我们研究了 MMP 表达、黑色素瘤细胞核仁直径、不同临床和组织学参数以及患者预后之间的相关性。研究人员对十七例鼻腔和口腔恶性黑色素瘤进行了研究。在石蜡切片上通过免疫组织化学方法评估 MMP2、MMP9、MMP13 和 MMP14 的表达，并通过计算机形态测量法和银染核仁直径进行测量。研究发现 MMP2 和 MMP14 表达与患者的预后存在显着相关性。平均 MMP2 表达低于 2 且低于 8000 mu m(2)/x20 高倍视野的患者的总体生存率更高 (P = .016)。在 MMP14 染色阴性的患者中，随访结束时的存活率为 38%，而 MMP 14 染色阳性的患者的存活率为 0 (P = .03)。还发现与发病年龄存在相关性； 66 岁以下患者的总体生存率高于 66 岁或以上患者 (P = .03)。最大核仁直径（MaxND）是另一个与临床结果显着相关的参数。与 MaxND 小于 8 μm 的组相比，MaxND 等于或大于 8 μm 的患者的预后明显较差 (P = .0009)。我们的初步研究表明，MMP2、MMP 14、MMP9 和 MaxND 可用作鼻腔和口腔恶性黑色素瘤患者的预后标志物。 (c) 2008 Elsevier Inc. 保留所有权利。
Sinonasal and oral malignant melanomas are rare malignancies accounting for less than 2% of all melanomas. Matrix metalloproteinases (MMPs) are proteolytic enzymes required for extracellular matrix degradation in a variety of physiological and pathologic processes including wound healing, embryogenesis, tumor invasion, and metastases. We studied the correlation between expression of MMPs, nucleolar diameter of melanoma cells, different clinical and histologic parameters, and patient's outcome. Seventeen cases of sinonasal and oral malignant melanoma were studied. The expression of MMP2, MMP9, MMP13, and MMP14 was assessed immunohistochemically on paraffinized sections and measured by computer morphometry as well as silver-stained nucleolar diameter. A significant correlation was found between MMP2 and MMP14 expression and patient's outcome. Greater overall survival was seen in patients with average MMP2 expression less 2 than 8000 mu m(2)/x20 high-power field (P = .016). In patients with negative MMP14 staining, survival rate by the end of the follow-up was 38% compared with patients with positive MMP 14 staining where survival rate was 0 (P = .03). A correlation with age at onset was also found; patients younger than 66 years had better overall survival rates than patients aged 66 years or older (P = .03). The maximal nucleolar diameter (MaxND) was another parameter that significantly correlated with clinical outcome. Patients with MaxND of 8 mu m or larger showed a significant worse prognosis compared with the group with MaxND less than 8 mu m (P = .0009). Our pilot study demonstrates that MMP2, MMP 14, MMP9, and MaxND might be used as prognostic markers in patients with sinonasal and oral malignant melanoma. (c) 2008 Elsevier Inc. All rights reserved.