EXPRESSION OF A MOUSE ZINC FINGER PROTEIN GENE IN BOTH SPERMATOCYTES AND OOCYTES DURING MEIOSIS
EXPRESSION OF A MOUSE ZINC FINGER PROTEIN GENE IN BOTH SPERMATOCYTES AND OOCYTES DURING MEIOSIS
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DOI：
10.1016/0012-1606(92)90120-6
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发表时间：
1992-10-01
影响因子：
2.7
通讯作者：
HIGASHINAKAGAWA, T
中科院分区：
文献类型：
--
作者：
NOCE, T;FUJIWARA, Y;HIGASHINAKAGAWA, T
为了鉴定减数分裂调控基因，我们从小鼠精母细胞cDNA文库中筛选了果蝇Krüppel基因典型表达的C2 H2型锌指结构蛋白的编码序列。分离出三个新的cDNA，它们被命名为CTfin 33、CTfin 51和CTfin 92。其中，CTfin 51为研究对象。推导的氨基酸序列显示其C-末端区域有7个锌指基序。北方杂交和原位杂交结果显示，CTfin 51 mRNA在粗线期以后的精母细胞和青春期前和成年男性早期圆形精子细胞中均有表达。用抗β-gal-CTfin 51融合蛋白的抗血清进行免疫细胞化学染色，结果显示，该融合蛋白定位于精母细胞和精子细胞的核内。粗线期以后的卵母细胞核也呈CTfin 51蛋白免疫反应阳性。免疫印迹显示，在睾丸和卵巢的蛋白质提取物中，在Mr 75，000处有一条带。这些结果表明，theCTfin 51基因编码的DNA结合调节蛋白功能与减数分裂在男性和女性配子发生。
In order to identify genes regulating meiosis, a mouse spermatocyte cDNA library was screened for sequences encoding proteins with C2H2-type zinc finger motifs which are typically expressed by theDrosophila Krüppelgene. Three new cDNAs were isolated, and they were designatedCTfin33,CTfin51, andCTfin92. Among them,CTfin51was selected for further study. The deduced amino acid sequence revealed seven zinc finger motifs in its C-terminal region. Northern blot andin situhybridization showedCTfin51mRNA expression in spermatocytes after the pachytene stage and in early stage round spermatids of prepuberal and adult males. Immunocytochemical staining with an antiserum against β-gal-CTfin51fusion protein was localized within nuclei of spermatocytes and spermatids. Oocyte nuclei after the pachytene stage also were immunoreactive forCTfin51protein. Immunoblots revealed a band atMr75,000 in protein extracts from the testis and the ovary. These results suggest that theCTfin51gene encodes a DNA-binding regulatory protein functionally associated with meiosis in both male and female gametogenesis.