MicroRNA-155 contributes to preeclampsia by down-regulating CYR61
MicroRNA-155 contributes to preeclampsia by down-regulating CYR61
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MicroRNA-155 通过下调 CYR61 导致先兆子痫
DOI：
10.1016/j.ajog.2010.01.057
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发表时间：
2010-05-01
影响因子：
9.8
通讯作者：
Hu, Yali
中科院分区：
文献类型：
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作者：
Zhang, Yanqing;Diao, Zhenyu;Hu, Yali
目的：本研究旨在通过miR-155研究子痫前期（preeclampsia，PE）发生发展的分子机制。研究设计：检测miR-155和富含半胱氨酸蛋白61（cysteine-rich protein 61，CYR 61）在PE和正常胎盘中的表达。使用体外转染验证CYR 61 3'非翻译区为miR-155的靶。结果：miR-155和CYR 61在PE组织中的表达水平呈负相关，miR-155表达上调，CYR 61表达下调。荧光素酶实验和CYR 61转染实验证实miR-155可有效靶向CYR 61的3'非翻译区。结论：本研究首次报道了miR-155的过表达通过靶向下调血管生成调节因子CYR 61，导致PE的病理改变。这一发现不仅表征了疾病的新机制，而且提供了潜在的治疗靶点。
OBJECTIVE: The aim of this study was to characterize the molecular mechanism of preeclampsia (PE) development through miR-155.STUDY DESIGN: PE and normal placentas were used to measure miR-155 and cysteine-rich protein 61 (CYR61) expression. CYR61 3' untranslated region was validated as the target of miR-155 using in vitro transfections. miR-155 and CYR61 expression levels were assessed by real-time reverse transcription polymerase chain reaction or Western blot.RESULTS: An inverse correlation was found between miR-155 and CYR61 expression levels, with miR-155 up-regulated and CYR61 down-regulated in PE tissues. Luciferase assays and CYR61 transfection assays experimentally validated that miR-155 efficiently targets the 3' untranslated region of CYR61.CONCLUSION: This study reported for the first time that overexpression of miR-155 contributes to PE development by targeting and down-regulating angiogenic regulating factor CYR61, leading to pathological alterations. This finding not only characterizes a new mechanism for the disease but also provides a potential therapeutic target.