Coalescence of Golgi fragments in microtubule-deprived living cells
Coalescence of Golgi fragments in microtubule-deprived living cells
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DOI：
10.1016/s0171-9335(99)80096-x
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发表时间：
1999-03-01
影响因子：
6.6
通讯作者：
Mironov, AA
中科院分区：
文献类型：
--
作者：
Polishchuk, RS;Polishchuk, EV;Mironov, AA
堆栈合并的过程中，一个重要的机制，高尔基体恢复有丝分裂，使用新的实验范式进行了研究。在微管被破坏的活细胞中，半乳糖基转移酶-GFP标记的高尔基体片段不断出现，生长，有时以1-2 μ m/min的速度移动，合并或逐渐减少和消失，高尔基体片段的周转率和合并率高度平衡，以保持每个细胞的高尔基体单位数恒定。此外，在细胞质光漂白后，一些高尔基岛出现，一些接受新的GalTase-GFP，从分散的高尔基碎片的边缘延伸的短小管经常在微堆之间形成桥梁，诱导它们的合并。聚结的频率也可以通过破坏肌动蛋白微丝来抑制。布雷菲德菌素A诱导高尔基体重新分布到内质网后，无论是小的高尔基体片段的生长还是它们的合并都导致了没有微管参与的区室化stark的形成。这些结果表明，这种孤立的高尔基体堆叠之间的合并是不依赖于微管的，因此可能是由膜管介导的。
The process of stack coalescence, an important mechanism of Golgi recovery from mitosis, was examined using novel experimental paradigms. In living cells with disrupted (by nocodazole) microtubules, galactosyl transferase-GFP-labelled Golgi fragments constantly appeared, grew sometimes moved with a speed of 1-2 mu m/min, coalesced or gradually diminished and disappeared, The rate of Golgi fragment turnover and coalescence was highly balanced to maintain a constant number of Golgi units per cell. Moreover some Golgi islands appear and some received new GalTase-GFP after photobleaching of cell cytoplasm, Short tubules extending from the rims of scattered Golgi fragments frequently formed bridges between ministacks, inducing their coalescence. The frequency of coalescence could also be inhibited by disruption of actin microfilaments. After the Golgi redistribution into endoplasmic reticulum induced by brefeldin A, either the growth of small Golgi fragments or their coalescence leads to compartmentalized stark formation without the participation of microtubules, These results demonstrate that this coalescence between isolated Golgi stacks is microtubule-independent and could thus be mediated by membranous tubules.