Bacterial Composition and Diversity in Breast Milk Samples from Mothers Living in Taiwan and Mainland China.
Bacterial Composition and Diversity in Breast Milk Samples from Mothers Living in Taiwan and Mainland China.
复制标题
DOI：
10.3389/fmicb.2017.00965
复制
发表时间：
2017
影响因子：
5.2
通讯作者：
Tsai YC
中科院分区：
文献类型：
--
作者：
Li SW;Watanabe K;Hsu CC;Chao SH;Yang ZH;Lin YJ;Chen CC;Cao YM;Huang HC;Chang CH;Tsai YC
母乳被广泛认为是婴儿健康生长和发育的最佳营养来源;它含有多种微生物群。在这里，我们描述了东亚妇女母乳中微生物群的多样性，并评估了分娩方式是否会影响健康母乳喂养母亲母乳中的微生物群。我们使用16 S rRNA焦磷酸测序法分析了从台湾和中国大陆六个地区（华中、华东、华北、东北、华南和西南）的133名健康母亲中收集的母乳样本中的微生物群。哺乳期（收集乳汁样本时的产后数月）和母体体重指数不影响母乳微生物群。来自七个地理区域的样品在家庭水平上的细菌组成差异显着。优势菌科依次为链球菌科（24.4%）、假单胞菌科（14.0%）、葡萄球菌科（12.2%）、乳杆菌科（6.2%）和草酸杆菌科（4.8%）。微生物谱被分为三个簇，由葡萄球菌科（簇1中的丰度：42.1%）、链球菌科（簇2：48.5%）或假单胞菌科（簇3：26.5%）驱动。属级微生物网络分析显示，革兰氏阳性葡萄球菌、链球菌和罗氏菌的丰度与革兰氏阴性不动杆菌、拟杆菌、盐单胞菌、草螺菌和假单胞菌的丰度呈负相关。来自剖腹产母亲（剖腹产组）的乳汁比来自阴道分娩母亲（阴道组）的乳汁具有显著更高的乳酸杆菌丰度（P < 0.05）和更高数量的独特未分类操作分类单位（OTU）（P < 0.001）。这些发现揭示了（i）在来自台湾和中国大陆的母亲的母乳中发现了微生物谱的地理差异，（ii）主要细菌家族链球菌科、葡萄球菌科和假单胞菌科是形成三个各自集群的关键组分，和（iii）在经历剖腹产的母亲的母乳中发现的独特OTU的数量显著多于那些阴道分娩的母亲。
Human breast milk is widely recognized as the best source of nutrients for healthy growth and development of infants; it contains a diverse microbiota. Here, we characterized the diversity of the microbiota in the breast milk of East Asian women and assessed whether delivery mode influenced the microbiota in the milk of healthy breast-feeding mothers. We profiled the microbiota in breast milk samples collected from 133 healthy mothers in Taiwan and in six regions of mainland China (Central, East, North, Northeast, South, and Southwest China) by using 16S rRNA pyrosequencing. Lactation stage (months postpartum when the milk sample was collected) and maternal body mass index did not influence the breast milk microbiota. Bacterial composition at the family level differed significantly among samples from the seven geographical regions. The five most predominant bacterial families were Streptococcaceae (mean relative abundance: 24.4%), Pseudomonadaceae (14.0%), Staphylococcaceae (12.2%), Lactobacillaceae (6.2%), and Oxalobacteraceae (4.8%). The microbial profiles were classified into three clusters, driven by Staphylococcaceae (abundance in Cluster 1: 42.1%), Streptococcaceae (Cluster 2: 48.5%), or Pseudomonadaceae (Cluster 3: 26.5%). Microbial network analysis at the genus level revealed that the abundances of the Gram-positive Staphylococcus, Streptococcus, and Rothia were negatively correlated with those of the Gram-negative Acinetobacter, Bacteroides, Halomonas, Herbaspirillum, and Pseudomonas. Milk from mothers who had undergone Caesarian section (C-section group) had a significantly higher abundance of Lactobacillus (P < 0.05) and a higher number of unique unclassified operational taxonomic units (OTUs) (P < 0.001) than that from mothers who had undergone vaginal delivery (vaginal group). These findings revealed that (i) geographic differences in the microbial profiles were found in breast milk from mothers living in Taiwan and mainland China, (ii) the predominant bacterial families Streptococcaceae, Staphylococcaceae, and Pseudomonadaceae were key components for forming three respective clusters, and (iii) a significantly greater number of unique OTUs was found in the breast milk from mothers who had undergone C-section than from those who had delivered vaginally.