Allocation of glycerolipids and glycerophospholipids from adults to eggs in Daphnia magna: Perturbations by compounds that enhance lipid droplet accumulation
Allocation of glycerolipids and glycerophospholipids from adults to eggs in Daphnia magna: Perturbations by compounds that enhance lipid droplet accumulation
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DOI：
10.1016/j.envpol.2018.07.102
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发表时间：
2018-11-01
影响因子：
8.9
通讯作者：
Barata, Carlos
中科院分区：
文献类型：
--
作者：
Fuertes, Inmaculada;Jordao, Rita;Barata, Carlos
化学物质对无脊椎动物脂质破坏性影响的分析受到我们对脂质代谢途径和完整脂质组的贫乏知识的限制。最近的研究表明，幼鱼和双酚A破坏了甲壳类动物大水蚤的脂滴动力学。本研究采用超高效液相色谱/飞行时间质谱（UHPLC/TOFMS）研究了稚虫（吡丙醚和法尼索酸甲酯）和双酚A如何破坏水蚤成虫体内甘油磷脂和甘油磷脂的动态及其在卵中的分配。脂质组学分析鉴定出234种脂质，分别对应于3类甘油脂、7类甘油磷脂和1类鞘脂，其中194种脂质随化学处理和时间的变化而变化。对照组和双酚A处理组的成年雌性有低水平的甘油三酯和高水平的甘油磷脂，而青少年处理组的成年雌性有高水平的甘油三酯和低水平的甘油磷脂。所产蛋的脂质含量呈相反趋势。由于幼鱼显著地减少了繁殖，雌性给它们的卵分配的甘油三酯比对照组少。有趣的是，暴露于双酚A环境下的雌性虽然产卵数量与对照组相当，但分配给卵子的甘油三酯却较少。薄层色谱分析证实了UHPLC/TOFMS的结果，并可以定性测定胆固醇，胆固醇也在暴露于幼鱼的雌性中积累。(C) 2018 Elsevier Ltd.版权所有。
Analysis of the disruptive effects of chemicals on lipids in invertebrates is limited by our poor knowledge of the lipid metabolic pathways and the complete lipidome. Recent studies shown that juvenoids and bisphenol A disrupted the dynamics of lipid droplets in the crustacean Daphnia magna. This study used ultra-high performance liquid chromatography/time-of-flight mass spectrometry (UHPLC/TOFMS) to study how juvenoids (pyriproxyfen and methyl farnesoate) and bisphenol A disrupt the dynamics of glycerophospholipids and glycerolipids in Daphnia adults and their allocation to eggs. Lipidomic analysis identified 234 individual lipids corresponding to three classes of glycerolipids, seven of glycerophospholipids, and one of sphingolipids, of which 194 changed according to the chemical treatments and time. Adult females in the control and bisphenol A treatment groups had low levels of triacylglycerols but high levels of glycerophospholipids, whereas those in the juvenoid treatment groups had high levels of triacylglycerols and low levels of glycerophospholipids. The opposite trend was observed for the lipid contents in the eggs produced. Because the juvenoids reduced reproduction dramatically, the females allocated less triacylglycerols to their eggs than the controls did. Interestingly, females exposed to bisphenol A allocated less triacylglycerols to their eggs despite producing a similar number of eggs as that of the controls. Thin-layer chromatography analyses confirmed the UHPLC/TOFMS results and allowed qualitative determination of cholesterol, which was also accumulated in females exposed to the juvenoids. (C) 2018 Elsevier Ltd. All rights reserved.