Microbial community analysis of field-grown soybeans with different nodulation phenotypes
Microbial community analysis of field-grown soybeans with different nodulation phenotypes
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DOI：
10.1128/aem.00833-08
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发表时间：
2008-09-01
影响因子：
4.4
通讯作者：
Minamisawa, Kiwamu
中科院分区：
文献类型：
--
作者：
Ikeda, Seishi;Rallos, Lynn Esther E.;Minamisawa, Kiwamu
与非大豆化（Nod（-））、野生型大豆化（Nod（-））和高大豆化（Nod（++））大豆的茎和根相关的微生物[Glycine max（L.）用核糖体基因间转录间隔区分析（RISA）和自动化RISA（ARISA）分析。茎样品的RISA没有检测到特异于结瘤表型的条带，而根样品的RISA显示结瘤表型的差异条带。荧光假单胞菌仅与Nod（+）大豆根相关。茄病镰刀菌与结瘤（Nod（+）和Nod（++））根稳定相关，在Nod（-）大豆中较少，而担子菌的丰度正好相反。系统发育分析表明，这些担子菌可能代表了木耳目中的一个根相关类群。主成分分析结果表明，茎中细菌群落结构与结球表型之间没有明显的关系。相反，根中的细菌和真菌群落结构都与根瘤菌表型有关。主成分分析进一步表明，根中细菌群落结构可根据结瘤表型分为Nod（-）、Nod（+）和Nod（++）3类。对大豆根样的微生物群落分析表明，与Nod（+）大豆相比，Nod（-）大豆与Nod（++）大豆的微生物群落更相似。
Microorganisms associated with the stems and roots of nonnodulated (Nod(-)), wild-type nodulated (Nod(-)), and hypernodulated (Nod(++)) soybeans [Glycine max (L.) Merril] were analyzed by ribosomal intergenic transcribed spacer analysis (RISA) and automated RISA (ARISA). RISA of stem samples detected no bands specific to the nodulation phenotype, whereas RISA of root samples revealed differential bands for the nodulation phenotypes. Pseudomonas fluorescens was exclusively associated with Nod(+) soybean roots. Fusarium solani was stably associated with nodulated (Nod(+) and Nod(++)) roots and less abundant in Nod(-) soybeans, whereas the abundance of basidiomycetes was just the opposite. The phylogenetic analyses suggested that these basidiomycetous fungi might represent a root-associated group in the Auriculariales. Principal-component analysis of the ARISA results showed that there was no clear relationship between nodulation phenotype and bacterial community structure in the stem. In contrast, both the bacterial and fungal community structures in the roots were related to nodulation phenotype. The principal-component analysis further suggested that bacterial community structure in roots could be classified into three groups according to the nodulation phenotype (Nod(-), Nod(+), or Nod(++)). The analysis of root samples indicated that the microbial community in Nod(-) soybeans was more similar to that in Nod(++) soybeans than to that in Nod(+) soybeans.