Bioimprinting for multiplex luminescent detection of deoxynivalenol and zearalenone
Bioimprinting for multiplex luminescent detection of deoxynivalenol and zearalenone
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DOI：
10.1016/j.talanta.2018.09.042
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发表时间：
2019-01-15
期刊：
影响因子：
6.1
通讯作者：
De Saeger, Sarah
中科院分区：
文献类型：
--
作者：
Beloglazova, Natalia;Lenain, Pieterjan;De Saeger, Sarah
首次提出了一种利用生物印迹蛋白同时定量测定同一点两种分析物的灵敏工具。BSA被选为生成两种化合物特异性结合位点的支架。以荧光二氧化硅包被的量子点为标记，实现了小麦和玉米中两种谷类真菌毒素--脱氧雪腐镰刀菌烯醇和玉米赤霉烯酮的多重免疫吸附检测。水溶性荧光纳米结构由包裹在二氧化硅壳层中的核/壳Cd-量子点组成，以确保它们的溶解性。通过在两个不同的波长扫描检测结果，可以同时检测霉菌毒素，因为两个QD@SiO_2标记的偶联物在光谱的不同部分具有荧光。该检测方法与快速提取技术相结合。玉米赤霉烯酮和脱氧雪腐镰刀菌烯醇在两种基质中的检出限分别为100和700µg·kg~(-1)。采用LC-MS/MS联用技术对所得结果进行了验证。
A sensitive tool for simultaneous quantitative determination of two analytes in a single spot with the use of a bioimprinted protein is presented for the first time. BSA is chosen as a scaffold for generation of binding sites specific towards two compounds. A multiplex immunosorbent assay for screening of two cereal-born mycotoxins, deoxynivalenol and zearalenone, in wheat and maize is realized with the use of fluorescent silica coated quantum dots as labels. Water-soluble fluorescent nanostructures consist of core/shell Cd-QDs enrobed in silica shells to ensure their solubility. The mycotoxins are simultaneously detected by scanning the assay outcome at two different wavelengths, since two QD@SiO2 labelled conjugates fluorescent in different parts of the spectrum. The assay is combined with a rapid extraction technique. The limits of detection for the simultaneous determination were 100 and 700 mu g kg(-1) in both matrices for zearalenone and deoxynivalenol, respectively. Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was used to confirm the obtained results.