Plant neopolyploidy and genetic background differentiate the microbiome of duckweed across a variety of natural freshwater sources
Plant neopolyploidy and genetic background differentiate the microbiome of duckweed across a variety of natural freshwater sources
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DOI：
10.1111/mec.17142
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发表时间：
2023-09-26
影响因子：
4.9
通讯作者：
Ashman，Tia-Lynn
中科院分区：
文献类型：
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作者：
Anneberg，Thomas J.;Turcotte，Martin M.;Ashman，Tia-Lynn
长期以来，全基因组复制因其在推动植物表型新奇方面的作用而受到重视，通常会改变生物体与非生物环境的界面。然而，直到最近，我们才开始研究多倍体如何影响植物与其他物种的相互作用，尽管生物生态位被预测为多倍体建立的主要决定因素之一。然而，我们缺乏关于多倍性如何影响定植植物的微生物类群的多样性和组成的信息，以及这是否是基因型依赖性的和在自然环境中可重复的。这些信息是了解微生物组是否有助于多倍体建立的第一步。因此，我们使用四对二倍体和合成同源四倍体测试了多倍体对水生植物多根螺旋藻细菌微生物组的多样性和组成的直接影响。在受控条件下，无菌植物接种池塘沃茨水收集从10个现场跨越广泛的环境梯度。同源四倍体的细菌分类和系统发育多样性比二倍体祖先高4%-11%。多倍性，沿着其与接种物来源和遗传谱系的相互作用，共同解释了微生物组组成的总变异的7%。此外，多倍体扩大了核心微生物组，同源四倍体除了与二倍体共有的55个细菌分类群外，还具有15个独特的细菌分类群。我们的研究结果表明，全基因组复制直接导致植物微生物组的新奇，重要的是，这种效应取决于多倍体的遗传祖先，并且在许多环境背景下都是普遍的。
Whole‐genome duplication has long been appreciated for its role in driving phenotypic novelty in plants, often altering the way organisms interface with the abiotic environment. Only recently, however, have we begun to investigate how polyploidy influences interactions of plants with other species, despite the biotic niche being predicted as one of the main determinants of polyploid establishment. Nevertheless, we lack information about how polyploidy affects the diversity and composition of the microbial taxa that colonize plants, and whether this is genotype‐dependent and repeatable across natural environments. This information is a first step towards understanding whether the microbiome contributes to polyploid establishment. We, thus, tested the immediate effect of polyploidy on the diversity and composition of the bacterial microbiome of the aquatic plantSpirodela polyrhizausing four pairs of diploids and synthetic autotetraploids. Under controlled conditions, axenic plants were inoculated with pond waters collected from 10 field sites across a broad environmental gradient. Autotetraploids hosted 4%–11% greater bacterial taxonomic and phylogenetic diversity than their diploid progenitors. Polyploidy, along with its interactions with the inoculum source and genetic lineage, collectively explained 7% of the total variation in microbiome composition. Furthermore, polyploidy broadened the core microbiome, with autotetraploids having 15 unique bacterial taxa in addition to the 55 they shared with diploids. Our results show that whole‐genome duplication directly leads to novelty in the plant microbiome and importantly that the effect is dependent on the genetic ancestry of the polyploid and generalizable over many environmental contexts.