No evidence for impacts to the molecular ecophysiology of ion or CO 2 regulation in tissues of selected surface-dwelling bivalves in the vicinity of a sub-seabed CO 2 release
No evidence for impacts to the molecular ecophysiology of ion or CO 2 regulation in tissues of selected surface-dwelling bivalves in the vicinity of a sub-seabed CO 2 release
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没有证据表明离子或 CO 2 调节对海底 CO 2 释放附近选定的表层双壳类动物组织的分子生态生理学有影响
DOI：
10.1016/j.ijggc.2014.10.001
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发表时间：
2015
影响因子：
3.9
通讯作者：
Pratt N
中科院分区：
文献类型：
--
作者：
Pratt N
虽然海底下碳捕获和储存(CCS)有可能从源头上消除相当大比例的人为二氧化碳排放，但有必要进行研究，以限制未来潜在的储气库气体泄漏对环境的影响。建立QICS项目(量化和监测地质碳储存的潜在生态系统影响)是为了增进我们对这些潜在影响的了解，并开发监测海底CCS水库的工具和最佳做法。许多海洋无脊椎动物暴露在环境中的二氧化碳含量增加，导致组织酸化，离子转运和碳酸氢盐缓冲都受到干扰。这些干扰可能导致下游效应，被视为易受影响的生物体的代谢抑制，损害特定物种在生态系统中的作用，甚至导致物种群体的局部灭绝。为了监测对表层底栖大型动物的潜在影响，在气体释放地点和参考地点部署了双壳类笼子(常见的贻贝，1758和大扇贝国王(Linnaeus，1758))--这两个地点都位于苏格兰欧班市的阿德穆克尼什湾。为了确定对分子生理学的影响，在125天的时间段内，在6个时间点对复制的个体进行了采样，这段时间跨越了实验的37天注射和恢复阶段。同时还从海湾内的一个参考地点采集了双壳类样本，以对比气体释放引起的生理变化与两个物种生理性能的自然变化。我们提供了编码关键离子和二氧化碳调节蛋白的基因转录变化的数据。在这两个物种中，都没有证据表明，在从二氧化碳气体释放地点收集的单个双壳类中，选定的碳酸氢酶(CAx基因)或钠钾ATPase的α亚单位(ATP1Agene)存在基因调控。在普通的肌肉中。仅有证据表明，编码不同类别碳酸氢酶的基因的表达随着时间的推移而变化。因此，由此得出的结论是，在这两个物种中，升高的pCO2羽流对离子调节基因转录的影响可以忽略不计。虽然本研究分析的数据不会阻碍海底下CCS作为一项气候缓解战略的继续发展，但需要进一步建模以预测更大或更长期泄漏的后果。还需要进一步的分析，以限制气体泄漏对底栖非动物群物种的潜在生理影响，并了解在穴居的牛心包虫病中记录的可能的回避行为的机制(Pennant，1777)。
Whilst sub-seabed Carbon Capture and Storage (CCS) has the potential to remove a significant proportion of anthropogenic CO2emissions at source, research is necessary to constrain the environmental impacts of potential future gas leaks from storage reservoirs. The QICS project (Quantifying and Monitoring Potential Ecosystem Impacts of Geological Carbons Storage) was established to improve our understanding of these potential impacts and to develop tools and best practice for monitoring sub-seabed CCS reservoirs. Exposure to increased environmental CO2has been shown to raise the tissue pCO2of many marine invertebrate species, leading to tissue acidosis and perturbations in both ion transport and bicarbonate buffering. These disturbances can cause downstream effects, seen as metabolic depression in susceptible organisms, compromising the role of particular species within an ecosystem and even causing the local extinction of species groups. To monitor the potential impact to surficial benthic megafauna, cages of bivalves (the common musselMytilus edulisLinnaeus, 1758 and the king scallopPecten maximus(Linnaeus, 1758)) were deployed at the gas release site and at a reference site—both within Ardmucknish Bay, Oban, Scotland. Replicate individuals were sampled at six time points over a 125-day period, which spanned both the 37-day injection and recovery phases of the experiment, in order to establish impacts to molecular physiology. Samples of bivalves were also simultaneously sampled from a reference site within the bay in order to contrast changes in physiology induced by the gas release with naturally variability in the physiological performance of both species. We present data on changes in the transcription of genes coding for key ionic and carbon dioxide regulatory proteins. There was no evidence of gene regulation of either selected carbonic anhydrases (CAxgenes) or the alpha subunit of sodium potassium ATPAses (ATP1Agenes) in individual bivalves collected from the CO2gas release site, in either species. In the common musselM. edulisthere was only evidence for changes with time in the expression of genes coding for different classes of carbonic anhydrase. It was therefore concluded that the effects of the plume of elevated pCO2on ion-regulatory gene transcription were negligible in both species. Whilst the analysed data from this current study do not constitute an impediment to the continued development of sub-seabed CCS as a climate mitigation strategy, further modelling is necessary to predict the consequences of larger or longer term leaks. Further analysis is also required in order to constrain the potential physiological impacts of gas leaks to benthic infaunal species and understand the mechanism of possible avoidance behaviour recorded in burrowing heart urchinsEchinocardium cordatum(Pennant, 1777).