Characterization of NDM-5 Carbapenemase-Encoding Gene (bla (NDM-5)) - Positive Multidrug Resistant Commensal Escherichia coli from Diarrheal Patients.
Characterization of NDM-5 Carbapenemase-Encoding Gene (bla (NDM-5)) - Positive Multidrug Resistant Commensal Escherichia coli from Diarrheal Patients.
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DOI：
10.2147/idr.s364526
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发表时间：
2022
影响因子：
3.9
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中科院分区：
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多重耐药肠杆菌科细菌引起许多严重的感染，导致住院时间延长，治疗费用增加和死亡率增加。在这项研究中，我们的特点blaNDM-5阳性多重耐药大肠杆菌（CE）分离自印度加尔各答的腹泻患者。从大肠埃希氏菌粪便中分离到3株CE菌株，对大肠埃希氏菌不同致病群均呈阴性。coli（DEC）。采用PCR检测碳青霉烯酶编码基因和其他抗菌药物耐药基因（ARG）的存在。通过质粒基因组测序研究了blaNDM-5的遗传排列。采用脉冲场凝胶电泳（PFGE）和多位点序列分型（MLST）方法对菌株进行遗传相关性分析。除粘菌素外，blaNDM-5阳性CE菌株对大多数抗生素均表现出耐药性。环丙沙星（>32 mg/L）和亚胺培南（8 mg/L）的MIC较高。分子分型结果显示，3株CE菌株分属于两个不同的ST（ST 101和ST 648），但PFGE分析显示它们的相似性为95%。ARG筛选显示CE菌株携带Int-1、blaTEM、blaCTX-M3、blaOXA-1、blaOXA-7、blaOXA-9、tetA、strA、aadA 1、aadB、sul 2、floR、mph（A）和aac（6 ′）-Ib-cr。在共轭实验中，转移频率范围为2.5×10−3 ~ 8.4 × 10 −5。blaNDM-5基因位于94-kb pNDM-TC-CE-89型质粒上，该质粒与具有IS 26-IS 30-blaNDM-5-bleMBL-trpF-dsbd-IS 91-dhps结构的IncPII质粒高度相似。据我们所知，这是第一份关于腹泻患者CE中涉及blaNDM-5基因的碳青霉烯类耐药性的报告。blaNDM-5基因在CE中的循环是令人担忧的，因为它有可能将blaNDM-5基因转移到其他肠道病原体。
The multidrug resistance Enterobacteriaceae cause many serious infections resulting in prolonged hospitalization, increased treatment charges and mortality rate. In this study, we characterized blaNDM-5-positive multidrug resistance commensal Escherichia coli (CE) isolated from diarrheal patients in Kolkata, India. Three CE strains were isolated from diarrheal stools, which were negative for different pathogroups of diarrheagenic E. coli (DEC). The presence of carbapenemases encoding genes and other antimicrobial resistance genes (ARGs) was detected using PCR. The genetic arrangement adjoining blaNDM-5 was investigated by plasmid genome sequencing. The genetic relatedness of the strains was determined by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) methods. In addition to colistin, the blaNDM-5-positive CE strains showed resistance to most of the antibiotics. Higher MICs were detected for ciprofloxacin (>32 mg/L) and imipenem (8 mg/L). Molecular typing revealed that three CE strains belonged to two different STs (ST 101 and ST 648) but they were 95% similar in the PFGE analysis. Screening for ARGs revealed that CE strains harbored Int-1, blaTEM, blaCTX-M3, blaOXA-1, blaOXA-7, blaOXA-9, tetA, strA, aadA1, aadB, sul2, floR, mph(A), and aac(6´)-Ib-cr. In conjugation experiment, transfer frequencies ranged from 2.5×10−3 to 8.4x10−5. The blaNDM-5 gene was located on a 94-kb pNDM-TC-CE-89 type plasmid, which is highly similar to the IncFII plasmid harboring an IS26-IS30-blaNDM-5-bleMBL-trpF-dsbd-IS91-dhps structure. To the best of our knowledge, this is the first report on carbapenem resistance involving the blaNDM-5 gene in CE from diarrheal patients. The circulation of blaNDM-5 gene in CE is worrisome, since it has the potential to transfer blaNDM-5 gene to other enteric pathogens.