Intracistronic complementation of the tetracycline resistance membrane protein of Tn10

Intracistronic complementation of the tetracycline resistance membrane protein of Tn10
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Tn10 的四环素抗性膜蛋白的顺反子内互补

DOI:
10.1128/jb.157.1.211-217.1984
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发表时间:
1984
影响因子:
3.2
通讯作者:
Levy
Levy
中科院分区:
生物学3区
文献类型:
--
作者:
M. Curiale;L. McMurry;B. Stuart;Levy

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Tn 10上四环素抗性结构基因区由tetA和tetB两个互补基团组成。S. Curiale和S. B。Levy,J. Bacteriol. 151:209-215,1982)。使用一系列的缺失突变体,我们已经确定,tetA区是450至600个碱基对长,tetB区,这是相邻的tetA,是600至750个碱基对长。tetA或tetB中的点突变影响了大肠杆菌大细胞中合成的可诱导内膜泰特蛋白的数量和大小。此外,这些区域的缺失导致合成适当较小的泰特蛋白。检测到约1,200个碱基的单个四环素诱导RNA,其与四环素抗性结构基因区同源。这些结果表明tetA和tetB互补区代表编码四环素抗性蛋白泰特的两个结构域的单个基因的两个部分。
The structural gene region for tetracycline resistance on Tn10 consists of two complementation groups, tetA and tetB (M. S. Curiale and S. B. Levy, J. Bacteriol. 151:209-215, 1982). Using a series of deletion mutants, we have determined that the tetA region is 450 to 600 base pairs long and that the tetB region, which is adjacent to tetA, is 600 to 750 base pairs long. Point mutations in either tetA or tetB affected the amount and size of the inducible inner-membrane Tet protein synthesized in Escherichia coli maxicells. Moreover, deletions in these regions led to the synthesis of an appropriately smaller Tet protein. A single tetracycline-inducible RNA of about 1,200 bases was detected that was homologous with the tetracycline resistance structural gene region. These results indicate that the tetA and tetB complementation regions represent two parts of a single gene encoding two domains of the tetracycline resistance protein Tet.
DOI: --
发表时间: 1983
期刊: The Journal of biological chemistry
影响因子: --
作者:
Jenness,DD;Schachman,HK
通讯作者: Schachman,HK
从转座子 Tn10 中纯化 TET 阻遏蛋白和 TET 操纵子并表征它们的相互作用。
DOI: --
发表时间: 1982
期刊: The Journal of biological chemistry
影响因子: --
作者:
Hillen,W;Klock,G;Kaffenberger,I;Wray,LV;Reznikoff,WS
通讯作者: Reznikoff,WS
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DOI: 10.1073/pnas.80.1.237
发表时间: 1983
影响因子: 11.1
作者:
Livneh,Z
通讯作者: Livneh,Z