Intracistronic complementation of the tetracycline resistance membrane protein of Tn10
Intracistronic complementation of the tetracycline resistance membrane protein of Tn10
复制标题
Tn10 的四环素抗性膜蛋白的顺反子内互补
DOI:
10.1128/jb.157.1.211-217.1984
复制
发表时间:
1984
影响因子:
3.2
通讯作者:
Levy
中科院分区:
文献类型:
--
作者:
M. Curiale;L. McMurry;B. Stuart;Levy
The structural gene region for tetracycline resistance on Tn10 consists of two complementation groups, tetA and tetB (M. S. Curiale and S. B. Levy, J. Bacteriol. 151:209-215, 1982). Using a series of deletion mutants, we have determined that the tetA region is 450 to 600 base pairs long and that the tetB region, which is adjacent to tetA, is 600 to 750 base pairs long. Point mutations in either tetA or tetB affected the amount and size of the inducible inner-membrane Tet protein synthesized in Escherichia coli maxicells. Moreover, deletions in these regions led to the synthesis of an appropriately smaller Tet protein. A single tetracycline-inducible RNA of about 1,200 bases was detected that was homologous with the tetracycline resistance structural gene region. These results indicate that the tetA and tetB complementation regions represent two parts of a single gene encoding two domains of the tetracycline resistance protein Tet.
DOI:
--
发表时间:
1983
期刊:
The Journal of biological chemistry
影响因子:
--
作者:
Jenness,DD;Schachman,HK
通讯作者:
Schachman,HK
DOI:
--
发表时间:
1982
期刊:
The Journal of biological chemistry
影响因子:
--
作者:
Hillen,W;Klock,G;Kaffenberger,I;Wray,LV;Reznikoff,WS
通讯作者:
Reznikoff,WS
DOI:
10.1073/pnas.80.1.237
发表时间:
1983
影响因子:
11.1
作者:
Livneh,Z
通讯作者:
Livneh,Z